CLC number: Q25; Q492
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2009-01-04
Cited: 1
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Da-lei ZHANG, Kai-ming WANG, Cai-qiao ZHANG. Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C[J]. Journal of Zhejiang University Science B, 2009, 10(2): 87-92.
@article{title="Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C",
author="Da-lei ZHANG, Kai-ming WANG, Cai-qiao ZHANG",
journal="Journal of Zhejiang University Science B",
volume="10",
number="2",
pages="87-92",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820133"
}
%0 Journal Article
%T Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C
%A Da-lei ZHANG
%A Kai-ming WANG
%A Cai-qiao ZHANG
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 2
%P 87-92
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820133
TY - JOUR
T1 - Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C
A1 - Da-lei ZHANG
A1 - Kai-ming WANG
A1 - Cai-qiao ZHANG
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 2
SP - 87
EP - 92
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820133
Abstract: The effect of ginsenosides on proliferation of type A spermatogonia was investigated in 7-day-old mice. spermatogonia were characterized by c-kit expression and cell proliferation was assessed by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA). After 72-h culture, Sertoli cells formed a confluent monolayer to which numerous spermatogonial colonies attached. spermatogonia were positive for c-kit staining and showed high proliferating activity by PCNA expression. ginsenosides (1.0~10 μg/ml) significantly stimulated proliferation of spermatogonia. Activation of protein kinase C (PKC) elicited proliferation of spermatogonia at 10−8 to 10−7 mol/L and the PKC inhibitor H7 inhibited this effect. Likewise, ginsenosides-stimulated spermatogonial proliferation was suppressed by combined treatment of H7. These results indicate that the proliferating effect of ginsenosides on mouse type A spermatogonia might be mediated by a mechanism involving the PKC signal transduction pathway.
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