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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.3 P.180~187


Spinal cord decompression reduces rat neural cell apoptosis secondary to spinal cord injury

Author(s):  Kan XU, Qi-xin CHEN, Fang-cai LI, Wei-shan CHEN, Min LIN, Qiong-hua WU

Affiliation(s):  Department of Orthopaedic Surgery; more

Corresponding email(s):   xukan@medmail.com.cn

Key Words:  Spinal cord injury, Decompression, Apoptosis, Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end labelling (TUNEL), Caspase-3, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X-protein (Bax)

Kan XU, Qi-xin CHEN, Fang-cai LI, Wei-shan CHEN, Min LIN, Qiong-hua WU. Spinal cord decompression reduces rat neural cell apoptosis secondary to spinal cord injury[J]. Journal of Zhejiang University Science B, 2009, 10(3): 180~187.

@article{title="Spinal cord decompression reduces rat neural cell apoptosis secondary to spinal cord injury",
author="Kan XU, Qi-xin CHEN, Fang-cai LI, Wei-shan CHEN, Min LIN, Qiong-hua WU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Spinal cord decompression reduces rat neural cell apoptosis secondary to spinal cord injury
%A Kan XU
%A Qi-xin CHEN
%A Fang-cai LI
%A Wei-shan CHEN
%A Min LIN
%A Qiong-hua WU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 3
%P 180~187
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820161

T1 - Spinal cord decompression reduces rat neural cell apoptosis secondary to spinal cord injury
A1 - Kan XU
A1 - Qi-xin CHEN
A1 - Fang-cai LI
A1 - Wei-shan CHEN
A1 - Min LIN
A1 - Qiong-hua WU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 3
SP - 180
EP - 187
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820161

Objective: To determine whether spinal cord decompression plays a role in neural cell apoptosis after spinal cord injury. Study design: We used an animal model of compressive spinal cord injury with incomplete paraparesis to evaluate neural cell apoptosis after decompression. apoptosis and cellular damage were assessed by staining with terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end labelling (TUNEL) and immunostaining for caspase-3, Bcl-2 and Bax. Methods: Experiments were conducted in male Sprague-Dawley rats (n=78) weighing 300~400 g. The spinal cord was compressed posteriorly at T10 level using a custom-made screw for 6 h, 24 h or continuously, followed by decompression by removal of the screw. The rats were sacrificed on Day 1 or 3 or in Week 1 or 4 post-decompression. The spinal cord was removed en bloc and examined at lesion site, rostral site and caudal site (7.5 mm away from the lesion). Results: The numbers of TUNEL-positive cells were significantly lower at the site of decompression on Day 1, and also at the rostral and caudal sites between Day 3 and Week 4 post-decompression, compared with the persistently compressed group. The numbers of cells between Day 1 and Week 4 were immunoreactive to caspase-3 and b-cell lymphoma-2 (Bcl-2)-associated X-protein (Bax), but not to Bcl-2, correlated with those of TUNEL-positive cells. Conclusion: Our results suggest that decompression reduces neural cell apoptosis following spinal cord injury.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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