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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.2 P.112~119


Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique

Author(s):  Manuel TORAL IBAÑ,EZ, Margarita CARU, Miguel A. HERRERA, Luis GONZALEZ, Luis M. MARTIN, Jorge MIRANDA, Rafael M. NAVARRO-CERRILLO

Affiliation(s):  Department of Forest Management, Faculty of Forestry; more

Corresponding email(s):   mtoral@uchile.cl

Key Words:  Sequoia sempervirens (D. Don) Endl., Random amplified polymorphic DNAs (PCR-RAPDs), Molecular markers, Clone assays, Chile, Genetic relationships

Manuel TORAL IBAÑEZ, Margarita CARU, Miguel A. HERRERA, Luis GONZALEZ, Luis M. MARTIN, Jorge MIRANDA, Rafael M. NAVARRO-CERRILLO. Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique[J]. Journal of Zhejiang University Science B, 2009, 10(2): 112~119.

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author="Manuel TORAL IBAÑEZ, Margarita CARU, Miguel A. HERRERA, Luis GONZALEZ, Luis M. MARTIN, Jorge MIRANDA, Rafael M. NAVARRO-CERRILLO",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique
%A Margarita CARU
%A Miguel A. HERRERA
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 2
%P 112~119
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820162

T1 - Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique
A1 - Manuel TORAL IBAÑ
A1 - EZ
A1 - Margarita CARU
A1 - Miguel A. HERRERA
A1 - Luis M. MARTIN
A1 - Jorge MIRANDA
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 2
SP - 112
EP - 119
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Y1 - 2009
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0820162

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geographic origin.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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