Full Text:   <1625>

CLC number: R15

On-line Access: 

Received: 2008-06-03

Revision Accepted: 2009-01-16

Crosschecked: 2009-01-06

Cited: 9

Clicked: 3962

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.4 P.280~284


Persistence of gentamicin residues in milk after the intramammary treatment of lactating cows for mastitis

Author(s):  Xun TAN, Ye-wen JIANG, Yi-jun HUANG, Song-hua HU

Affiliation(s):  Department of Veterinary Medicine, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   songhua@zju.edu.cn

Key Words:  Gentamicin, Mastitis, Intramammary infusion, Residue

Xun TAN, Ye-wen JIANG, Yi-jun HUANG, Song-hua HU. Persistence of gentamicin residues in milk after the intramammary treatment of lactating cows for mastitis[J]. Journal of Zhejiang University Science B, 2009, 10(4): 280~284.

@article{title="Persistence of gentamicin residues in milk after the intramammary treatment of lactating cows for mastitis",
author="Xun TAN, Ye-wen JIANG, Yi-jun HUANG, Song-hua HU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Persistence of gentamicin residues in milk after the intramammary treatment of lactating cows for mastitis
%A Xun TAN
%A Ye-wen JIANG
%A Yi-jun HUANG
%A Song-hua HU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 4
%P 280~284
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820198

T1 - Persistence of gentamicin residues in milk after the intramammary treatment of lactating cows for mastitis
A1 - Xun TAN
A1 - Ye-wen JIANG
A1 - Yi-jun HUANG
A1 - Song-hua HU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 4
SP - 280
EP - 284
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820198

This study was designed to investigate persistence of gentamicin residues in milk after the intramammary treatment of lactating cows for mastitis. Milk samples were collected at a 1-d interval after the last administration from 34 individual cows that had received intramammary infusions of gentamicin. The doses and treatment times evaluated in this study represented those that have been applied by veterinarians in practice. The tetrazolium chloride assay was used to determine whether there were significant residues of the antibiotic in the samples. Persistence of detectable drug residues in milk from 33 cows (28 cows, ≤6 infusions at ≤0.7 g gentamicin; and 5 cows, 2 infusions at 0.8 g gentamicin) did not exceed 5 d; but 1 cow (5 infusions at 0.8 g gentamicin) had detectable residues in its milk for 9 d. Our results suggest that a 5-d milk withdrawal period might be insufficient to secure the clearance of the contamination of gentamicin, because treatment times and dosages contribute to the antibiotic clearance. A larger scale of samples are needed for further investigations.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Anderson, K.L., Moats, W.A., Rushing, J.E., O′Carroll, J.M., 1998. Detection of milk antibiotic residues by use of screening tests and liquid chromatography after intramammary administration of amoxicillin or penicillin G in cows with clinical mastitis. Am. J. Vet. Res., 59(9): 1096-1100.

[2] Bai, J., Liang, W., Sun, H., Cao, J., Huang, S., 2005. Examination of residue of antibiotics in pure milk from retail market. Beverage Fast Frozen Food Ind., 11(3):34-37 (in Chinese).

[3] Brady, M.S., Katz, S.E., 1988. Antibiotic/antimicrobial residues in milk. J. Food Prot., 51(1):8-11.

[4] Bureau of Husbandry and Veterinary, Ministry of Agriculture of the People’s Republic of China, 2003. The maximal limits of veterinary medicine in animal food approved by the Ministry of Agriculture of China. Chin. J. Vet. Drug., 37(3):5-11 (in Chinese).

[5] Burrows, G.E., Parto, P.B., Martin, B.B., 1987. Comparative pharmacokinetics of gentamicin, neomycin and oxytetracycline in newborn calves. J. Vet. Pharmacol. Ther., 10(1):54-63.

[6] Dayan, A.D., 1993. Allergy to antimicrobial residues in food: assessment of the risk to man. Vet. Microbiol., 35(3-4): 213-226.

[7] Deng, D.Y., Yu, S.B., Liang, J.T., Chen, S.L., Yang, Q., 2004. Investigation into the residual status of antibiotics in milk and milk powder in Foshan City, Guangdong Province. Chin. Trop. Med., 4(3):470-471 (in Chinese).

[8] Dewdney, J.M., Maes, L., Raynaud, J.P., Blanc, F., Scheid, J.P., Jackson, T., Lens, S., Verschueren, C., 1991. Risk assessment of antibiotic residues of beta-lactams and macrolides in food products with regard to their immuno-allergic potential. Food Chem. Toxicol., 29(7):477-483.

[9] Frazier, D.L., Aucoin, D.P., Riviere, J.E., 1988. Gentamicin pharmacokinetics and nephrotoxicity in naturally acquired and experimentally induced disease in dogs. J. Am. Vet. Med. Assoc., 192(1):57-63.

[10] Garg, S.K., Verma, S.P., Garg, B.D., 1991. Disposition kinetics of gentamicin in buffalo calves (Bubalus bubalis) following single intravenous administration. J. Vet. Pharmacol. Ther., 14(3):335-340.

[11] He, J.H., Wang, Y.F., 2007. Determination of antibiotic residues in pure milk with TTC and ELISA tests. J. Anhui Agric. Sci., 35(9):2576-2577 (in Chinese).

[12] Hillerton, J.E., Halley, B.I., Neaves, P., Rose, M.D., 1999. Detection of antimicrobial substances in individual cow and quater milk samples using Delvotest microbial. J. Dairy Sci., 82(4):704-711.

[13] McEwen, S.A., Meek, A.H., Black, W.D., 1991. A dairy farm survey of antibiotic treatment practices, residue control methods and associations with inhibitors in milk. J. Food Prot., 54(6):454-459.

[14] Ministry of Health of the People’s Republic of China, Standardization Administration of the People’s Republic of China, 2003. National Standards of the People’s Republic of China: Microbiological Examination of Food Hygiene- Examination of Residue of Antibiotics in Fresh Milk (GB/T 4789.27-2003). Standards Press of China, Beijing, China, p.189-190 (in Chinese).

[15] Moats, W.A., 1988. Inactivation of antibiotics by heating in foods and other substrates: a review. J. Food Prot., 51(6):491-497.

[16] Moretain, J.P., Boisseau, J., 1993. Elimination of aminoglycoside antibiotics in milk following intramammary administration. Vet. Q., 15(3):112-117.

[17] Nijsten, R., London, N., van de Bogaard, A., Stobberingh, E., 1996. Antibiotic resistance among Escherichia coli isolated from faecal samples of pig farmer and pig. J. Antimicrob. Chemother., 37(6):1131-1140.

[18] Payne, M.A., Baynes, R.E., Sundlof, S.F., Craigmill, A., Webb, A.I., Riviere, J.E., 1999. Drugs prohibited from extralabel use in food animals. J. Am. Vet. Med. Assoc., 215(1): 28-32.

[19] Pedersoli, W.M., Jackson, J., Frobish, R.A., 1995. Depletion of gentamicin in the milk of Holstein cows after single and repeated intramammary and parenteral treatments. J. Vet. Pharmacol. Ther., 18(6):457-463.

[20] Popelka, P., Nagy, J., Popelka, P., Sokol, J., Hajurka, J., Cabadaj, R., Marcincak, S., Bugarsky, A., 2003. Comparison of various methods for penicillin residue detection in cow milk after intramammary and parenteral treatment. Bull. Vet. Inst. Pulawy, 47(1):203-209.

[21] Ramsden, R.T., Wilson, P., Gibson, W.P.R., 1980. Immediate effects of intravenous tobramycin and gentamicin on human cochlear function. J. Laryngol. Otol., 94(5): 521-531.

[22] Schenck, F.J., Callery, P.S., 1998. Chromatographic methods of analysis of antibiotics in milk. J. Chromatogr. A, 812(1-2):99-109.

[23] Smith, G.W., Gehring, R., Craigmill, A.L., Webb, A.I., Riviere, J.E., 2005. Extralabel intramammary use of drugs in dairy cattle. J. Am. Vet. Med. Assoc., 226(12):1994-1996.

[24] Tan, X., Huang, Y.J., Jiang, Y.W., Hu, S.H., 2007. Persistence of oxytetracycline residues in milk after the intrauterine treatment of lactating cows for endometritis. Vet. Rec., 161(17):585-586.

[25] Thibault, N., Grenier, L., Simard, M., Bergeron, M.G., Beauchamp, D., 1994. Attenuation by daptomycin of gentamicin-induced experimental nephrotoxicity. Antimicrob. Agents Chemother., 38(5):1027-1035.

[26] van den Bogaard, A.E., London, N., Driessen, C., Stobberingh, E.E., 2001. Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. J. Antimicrob. Chemother., 47(6):763-771.

[27] Wang, F., Cao, L.T., Hu, S.H., 2006. In vitro inhibitory effect of nisin on major pathogenic bacteria of bovine mastitis. Chin. J. Vet. Drug, 40(10):12-16 (in Chinese).

[28] Weir, B.A., Mdzumdar, D.C., 1994. Aminoglycoside nephrotoxicity following single-dose cystoscopy prophylaxis. Ann. Pharmacother., 28(2):199-201.

[29] Wilson, D.J., Sears, P.M., Hutchinson, L.J., 1998. Dairy producer attitudes and farm practices used to reduce the likelihood of antibiotic residues in milk and dairy beef: a five state survey. Vet. Clin. North Am. Large Anim. Pract., 19(5):24-30.

[30] Wu, G.J., Sheng, H., Zhang, H.W., Zhou, D.G., Fan, H., Ge, Z.H., Yan, X.F., Yan, X., 2002. Assay on residue of antibiotics in sterilized pure milk from market. J. Beijing Agric. Coll., 17(2):33-37 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE