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Received: 2008-07-28

Revision Accepted: 2008-10-22

Crosschecked: 2009-04-08

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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.5 P.380~390

http://doi.org/10.1631/jzus.B0820230


Astragalus mongholicus ameliorates renal fibrosis by modulating HGF and TGF-β in rats with unilateral ureteral obstruction


Author(s):  Chuan ZUO, Xi-sheng XIE, Hong-yu QIU, Yao DENG, Da ZHU, Jun-ming FAN

Affiliation(s):  Department of Nephrology, West China Hospital of Sichuan University, Chengdu 610041, China

Corresponding email(s):   zcsiren@163.com, fanjunming2008@163.com

Key Words:  Astragalus mongholicus (AM), Myofibroblast, Transforming growth factor-&beta, 1 (TGF-&beta, 1), Renal interstitial fibrosis, Hepatocyte growth factor (HGF)


Chuan ZUO, Xi-sheng XIE, Hong-yu QIU, Yao DENG, Da ZHU, Jun-ming FAN. Astragalus mongholicus ameliorates renal fibrosis by modulating HGF and TGF-β in rats with unilateral ureteral obstruction[J]. Journal of Zhejiang University Science B, 2009, 10(5): 380~390.

@article{title="Astragalus mongholicus ameliorates renal fibrosis by modulating HGF and TGF-β in rats with unilateral ureteral obstruction",
author="Chuan ZUO, Xi-sheng XIE, Hong-yu QIU, Yao DENG, Da ZHU, Jun-ming FAN",
journal="Journal of Zhejiang University Science B",
volume="10",
number="5",
pages="380~390",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820230"
}

%0 Journal Article
%T Astragalus mongholicus ameliorates renal fibrosis by modulating HGF and TGF-β in rats with unilateral ureteral obstruction
%A Chuan ZUO
%A Xi-sheng XIE
%A Hong-yu QIU
%A Yao DENG
%A Da ZHU
%A Jun-ming FAN
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 5
%P 380~390
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820230

TY - JOUR
T1 - Astragalus mongholicus ameliorates renal fibrosis by modulating HGF and TGF-β in rats with unilateral ureteral obstruction
A1 - Chuan ZUO
A1 - Xi-sheng XIE
A1 - Hong-yu QIU
A1 - Yao DENG
A1 - Da ZHU
A1 - Jun-ming FAN
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 5
SP - 380
EP - 390
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820230


Abstract: 
Astragalus mongholicus (AM) derived from the dry root of Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is a widely used traditional Chinese medicine. The present study investigated the potential role of AM on renal fibrosis on a rat model of unilateral ureteral obstruction (UUO). We divided 48 Sprague-Dawley rats randomly into 4 groups: sham-operated group (Sham), untreated UUO group, AM-treated (10 g/(kg∙d)) UUO group, and losartan-treated (20 mg/(kg∙d)) UUO group as positive control. Haematoxylin & eosin (HE) and Masson staining were used to study the dynamic histological changes of the kidneys 7 and 14 d after operation. The expressions of fibronectin (FN), type I collagen (colI), hepatocyte growth factor (HGF), transforming growth factor-&beta;1 (TGF-&beta;1), and α-smooth muscle actin (α-SMA) were analyzed by real-time polymerase chain reaction (PCR), immunohistochemistry staining, and Western blot. Results show that, similar to losartan, AM alleviated the renal damage and decreased the deposition of FN and colI from UUO by reducing the expressions of TGF-β1 and α-SMA (P<0.05), whereas HGF increased greatly with AM treatment (P<0.05). Our findings reveal that AM could retard the progression of renal fibrosis. The renoprotective effect of AM might be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-β1 expression.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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