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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.3 P.188~192

http://doi.org/10.1631/jzus.B0820240


Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells


Author(s):  Zhi YANG, Miao LIU, Yin-gang ZHANG, Xiong GUO, Peng XU

Affiliation(s):  Department of Orthopaedics, the First Affiliated Hospital, School of Medicine; more

Corresponding email(s):   xgcgfd@126.com

Key Words:  Negative pressure, Bone marrow-derived stroma cells (BMSCs), Osteogenesis


Zhi YANG, Miao LIU, Yin-gang ZHANG, Xiong GUO, Peng XU. Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells[J]. Journal of Zhejiang University Science B, 2009, 10(3): 188~192.

@article{title="Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells",
author="Zhi YANG, Miao LIU, Yin-gang ZHANG, Xiong GUO, Peng XU",
journal="Journal of Zhejiang University Science B",
volume="10",
number="3",
pages="188~192",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820240"
}

%0 Journal Article
%T Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells
%A Zhi YANG
%A Miao LIU
%A Yin-gang ZHANG
%A Xiong GUO
%A Peng XU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 3
%P 188~192
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820240

TY - JOUR
T1 - Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells
A1 - Zhi YANG
A1 - Miao LIU
A1 - Yin-gang ZHANG
A1 - Xiong GUO
A1 - Peng XU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 3
SP - 188
EP - 192
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820240


Abstract: 
Objective: We investigated the effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells (BMSCs) in vitro. Methods: BMSCs were isolated from adult marrow donated by a hip osteoarthritis patient with prosthetic replacement and cultured in vitro. The third passage cells were divided into negative pressure treatment group and control group. The treatment group was induced by negative pressure intermittently (pressure: 50 kPa, 30 min/times, and twice daily). The control was cultured in conventional condition. The osteogenesis of BMSCs was examined by phase-contrast microscopy, the determination of alkaline phosphatase (ALP) activities, and the immunohistochemistry of collagen type I. The mRNA expressions of osteoprotegerin (OPG) and osteoprotegerin ligand (OPGL) in BMSCs were analyzed by real-time polymerase chain reaction (PCR). Results: BMSCs showed a typical appearance of osteoblast after 2 weeks of induction by intermittent negative pressure, the activity of ALP increased significantly, and the expression of collagen type I was positive. In the treatment group, the mRNA expression of OPG increased significantly (P<0.05) and the mRNA expression of OPGL decreased significantly (P<0.05) after 2 weeks, compared with the control. Conclusion: Intermittent negative pressure could promote osteogenesis in human BMSCs in vitro.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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