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Received: 2009-06-24

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Crosschecked: 2009-12-31

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.2 P.109-114

http://doi.org/10.1631/jzus.B0900176


Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China


Author(s):  Hui ZHANG, Xin-ying MA, Ya-juan QIAN, Xue-ping ZHOU

Affiliation(s):  Key Laboratory of Molecular Biology of Crop Pathogens and Insects of Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   zzhou@zju.edu.cn

Key Words:  Papaya leaf curl China virus (PaLCuCNV), Tomato, Infectivity


Hui ZHANG, Xin-ying MA, Ya-juan QIAN, Xue-ping ZHOU. Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China[J]. Journal of Zhejiang University Science B, 2010, 11(2): 109-114.

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%A Xin-ying MA
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T1 - Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China
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A1 - Xue-ping ZHOU
J0 - Journal of Zhejiang University Science B
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DOI - 10.1631/jzus.B0900176


Abstract: 
papaya leaf curl China virus (PaLCuCNV) was previously reported as a distinct begomovirus infecting papaya in southern China. Based on molecular diagnostic survey, 13 PaLCuCNV isolates were obtained from tomato plants showing leaf curl symptoms in Henan and Guangxi Provinces of China. Complete nucleotide sequences of 5 representative isolates (AJ558116, AJ558117, AJ704604, FN256260, and FN297834) were determined to be 2738–2751 nucleotides, which share 91.7%–97.9% sequence identities with PaLCuCNV isolate G2 (AJ558123). DNA-β was not found to be associated with PaLCuCNV isolates. To investigate the infectivity of PaLCuCNV, an infectious clone of PaLCuCNV-[CN:HeNZM1] was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants, which induced severe leaf curling and crinkling symptoms in these plants. Southern blot analysis and polymerase chain reaction (PCR) indicated a systemic infection of test plants by the agro-infectious clone.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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