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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.4 P.267-274


Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury

Author(s):  An-lu Dai, Li-hua Fan, Feng-jiang Zhang, Mei-juan Yang, Jing Yu, Jun-kuan Wang, Tao Fang, Gang Chen, Li-na Yu, Min Yan

Affiliation(s):  Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Department of Anesthesiology, Lishui People's Hospital, Lishui 323600, China, Department of Anesthesiology, Jinhua People's Hospital, Jinhua 321300, China

Corresponding email(s):   yanminnina@hotmail.com

Key Words:  Inhalation anesthetics, Sevoflurane, Postconditioning, Preconditioning, Ischemia-reperfusion injury, Myocardial stunning

An-lu Dai, Li-hua Fan, Feng-jiang Zhang, Mei-juan Yang, Jing Yu, Jun-kuan Wang, Tao Fang, Gang Chen, Li-na Yu, Min Yan. Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury[J]. Journal of Zhejiang University Science B, 2010, 11(4): 267-274.

@article{title="Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury",
author="An-lu Dai, Li-hua Fan, Feng-jiang Zhang, Mei-juan Yang, Jing Yu, Jun-kuan Wang, Tao Fang, Gang Chen, Li-na Yu, Min Yan",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury
%A An-lu Dai
%A Li-hua Fan
%A Feng-jiang Zhang
%A Mei-juan Yang
%A Jing Yu
%A Jun-kuan Wang
%A Tao Fang
%A Gang Chen
%A Li-na Yu
%A Min Yan
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 4
%P 267-274
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900390

T1 - Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury
A1 - An-lu Dai
A1 - Li-hua Fan
A1 - Feng-jiang Zhang
A1 - Mei-juan Yang
A1 - Jing Yu
A1 - Jun-kuan Wang
A1 - Tao Fang
A1 - Gang Chen
A1 - Li-na Yu
A1 - Min Yan
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 4
SP - 267
EP - 274
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900390

Ischemic preconditioning and postconditioning distinctly attenuate ventricular arrhythmia after ischemia without affecting the severity of myocardial stunning. Therefore, we report the effects of sevoflurane preconditioning and postconditioning on stunned myocardium in isolated rat hearts. Isolated rat hearts were underwent 20 min of global ischemia and 40 min of reperfusion. After an equilibration period (20 min), the hearts in the preconditioning group were exposed to sevoflurane for 5 min and next washout for 5 min before ischemia. Hearts in the sevoflurane postconditioning group underwent equilibration and ischemia, followed immediately by sevoflurane exposure for the first 5 min of reperfusion. The control group received no treatment before and after ischemia. Left ventricular pressure, heart rate, coronary flow, electrocardiogram, and tissue histology were measured as variables of ventricular function and cellular injury, respectively. There was no significant difference in the duration of reperfusion ventricular arrhythmias between control and sevoflurane preconditioning group (P=0.195). The duration of reperfusion ventricular arrhythmias in the sevoflurane postconditioning group was significantly shorter than that in the other two groups (P<0.05). ±(dP/dt)max in the sevoflurane preconditioning group at 5, 10, 15, 20, and 30 min after reperfusion was significantly higher than that in the control group (P<0.05), and there were no significant differences at 40 min after reperfusion among the three groups (P>0.05). As expected, for a 20-min general ischemia, infarct size in heart slices determined by 2,3,5-triphenyltetrazolium chloride staining among the groups was not obvious. sevoflurane postconditioning reduces reperfusion arrhythmias without affecting the severity of myocardial stunning. In contrast, sevoflurane preconditioning has no beneficial effects on reperfusion arrhythmias, but it is in favor of improving ventricular function and recovering myocardial stunning. sevoflurane preconditioning and postconditioning may be useful for correcting the stunned myocardium.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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