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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.5 P.357-365


Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide

Author(s):  Abdul Hamid Hafizah, Zakaria Zaiton, Amom Zulkhairi, Adenan Mohd Ilham, Megat Mohd Nordin Nor Anita, Abdullah Mahdy Zaleha

Affiliation(s):  Department of Physiology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Raja Muda Aziz, 50300 Kuala Lumpur, Malaysia, Department of Obstetrics and Gynecology, Universiti Kebangsaan Malaysia Medical Centre, Cheras, 56000 Kuala Lumpur, Malaysia, Department of Human Anatomy, Division of Physiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400 Selangor, Malaysia, Forest Research Institute of Malaysia, Kepong, 52109 Selangor, Malaysia

Corresponding email(s):   zaitonukm@gmail.com

Key Words:  Piper sarmentosum, Human umbilical vein endothelial cells (HUVECs), Malondialdehyde, Oxidative stress, Antioxidant enzymes

Abdul Hamid Hafizah, Zakaria Zaiton, Amom Zulkhairi, Adenan Mohd Ilham, Megat Mohd Nordin Nor Anita, Abdullah Mahdy Zaleha. Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide[J]. Journal of Zhejiang University Science B, 2010, 11(5): 357-365.

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author="Abdul Hamid Hafizah, Zakaria Zaiton, Amom Zulkhairi, Adenan Mohd Ilham, Megat Mohd Nordin Nor Anita, Abdullah Mahdy Zaleha",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide
%A Abdul Hamid Hafizah
%A Zakaria Zaiton
%A Amom Zulkhairi
%A Adenan Mohd Ilham
%A Megat Mohd Nordin Nor Anita
%A Abdullah Mahdy Zaleha
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 5
%P 357-365
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900397

T1 - Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide
A1 - Abdul Hamid Hafizah
A1 - Zakaria Zaiton
A1 - Amom Zulkhairi
A1 - Adenan Mohd Ilham
A1 - Megat Mohd Nordin Nor Anita
A1 - Abdullah Mahdy Zaleha
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 5
SP - 357
EP - 365
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900397

Endothelial cell death due to increased reactive oxygen species (ROS) may contribute to the initial endothelial injury, which promotes atherosclerotic lesion formation. Piper sarmentosum (PS), a natural product, has been shown to have an antioxidant property, which is hypothesized to inhibit production of ROS and prevent cell injury. Thus, the present study was designed to determine the effects of PS on the hydrogen peroxide (H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells (HUVECs). In this experiment, HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation (LSGS). HUVECs were treated with various concentrations of H2O2 (0–1000 µmol/L) and it was observed that 180 µmol/L H2O2 reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Using the above concentration as the positive control, the H2O2-induced HUVECs were concomitantly treated with various concentrations (100, 150, 250 and 300 µg/ml) of three different extracts (aqueous, methanol and hexane) of PS. malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) levels showed a significant increase (P<0.05) in HUVECs compared to the negative control. However, PS extracts showed a protective effect on HUVECs from H2O2-induced cell apoptosis with a significant reduction in MDA, SOD, CAT and GPX levels (P<0.05). Furthermore, PS had exhibited ferric reducing antioxidant power with its high phenolic content. Hence, it was concluded that PS plays a beneficial role in reducing oxidative stress in H2O2-induced HUVECs.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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