CLC number: Q93
On-line Access: 2010-06-02
Received: 2009-12-11
Revision Accepted: 2010-04-01
Crosschecked: 2010-05-04
Cited: 11
Clicked: 5890
Xiu-ju Du, Jing-song Zhang, Yan Yang, Qing-jiu Tang, Wei Jia, Ying-jie Pan. Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies[J]. Journal of Zhejiang University Science B, 2010, 11(6): 437-442.
@article{title="Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies",
author="Xiu-ju Du, Jing-song Zhang, Yan Yang, Qing-jiu Tang, Wei Jia, Ying-jie Pan",
journal="Journal of Zhejiang University Science B",
volume="11",
number="6",
pages="437-442",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900402"
}
%0 Journal Article
%T Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies
%A Xiu-ju Du
%A Jing-song Zhang
%A Yan Yang
%A Qing-jiu Tang
%A Wei Jia
%A Ying-jie Pan
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 6
%P 437-442
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900402
TY - JOUR
T1 - Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies
A1 - Xiu-ju Du
A1 - Jing-song Zhang
A1 - Yan Yang
A1 - Qing-jiu Tang
A1 - Wei Jia
A1 - Ying-jie Pan
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 6
SP - 437
EP - 442
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900402
Abstract: Ultrafiltration and a series of chromatographic steps were used to isolate and purify polysaccharides from Tremella aurantialba fruit bodies. Three crude fractions (TAP50w, TAP10–50w, and TAP1–10w), five semi-purified fractions (TAPA–TAPE), and one purified fraction (TAPA1) were obtained. A sulfated derivative of TAPA1 (TAPA1-s) was prepared by chemical modification. The immunostimulating activity of the polysaccharide fractions in vitro was determined using the mouse spleen lymphocyte proliferation assay. Of the three crude fractions tested, cell proliferation rates were increased most by TAP50w. Furthermore, TAPA1-s was markedly more stimulatory than TAPA1, indicating that sulfonation was an effective way to enhance the immunostimulating activity of polysaccharide.
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