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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.3 P.190-199

http://doi.org/10.1631/jzus.B0910350


Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings


Author(s):  Zhuo-mei Chen, Ying-xu Chen, Guo-jian Du, Xi-lin Wu, Feng Li

Affiliation(s):  College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029, China, Zhejiang Forestry Academy, Hangzhou 310023, China, Department of Geography, Minjiang University, Fuzhou 350108, China

Corresponding email(s):   zhuomeichen@163.com, yxchen@zju.edu.cn

Key Words:  Cinnamomum camphora, Fumigation, Growth, Chlorophyll content, Chlorophyll fluorescence, Antioxidant, Lipid peroxidation


Zhuo-mei Chen, Ying-xu Chen, Guo-jian Du, Xi-lin Wu, Feng Li. Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings[J]. Journal of Zhejiang University Science B, 2010, 11(3): 190-199.

@article{title="Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings",
author="Zhuo-mei Chen, Ying-xu Chen, Guo-jian Du, Xi-lin Wu, Feng Li",
journal="Journal of Zhejiang University Science B",
volume="11",
number="3",
pages="190-199",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0910350"
}

%0 Journal Article
%T Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings
%A Zhuo-mei Chen
%A Ying-xu Chen
%A Guo-jian Du
%A Xi-lin Wu
%A Feng Li
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 3
%P 190-199
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0910350

TY - JOUR
T1 - Effects of 60-day NO2 fumigation on growth, oxidative stress and antioxidative response in Cinnamomum camphora seedlings
A1 - Zhuo-mei Chen
A1 - Ying-xu Chen
A1 - Guo-jian Du
A1 - Xi-lin Wu
A1 - Feng Li
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 3
SP - 190
EP - 199
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0910350


Abstract: 
Objective: To study the oxidative stress and antioxidative response of Cinnamomum camphora seedlings exposed to nitrogen dioxide (NO2) fumigation. Methods: Measurements were made up of the growth, chlorophyll content, chlorophyll fluorescence, antioxidant system and lipid peroxidation of one-year-old C. camphora seedlings exposed to NO2 (0.1, 0.5, and 4 μl/L) fumigation in open top chambers over a period of 60 d. Results: After the first 30 d, 0.5 and 4.0 μl/L NO2 showed insignificant effects on the growth of C. camphora seedlings. However, exposure to 0.5 and 4.0 μl/L NO2 for 15 d significantly reduced their chlorophyll content (P<0.05), enhanced their malondialdehyde (MDA) content and superoxide dismutase (SOD) activity (P<0.05), and also significantly reduced the maximal quantum yield of PSII in the dark [the ratio of variable fluorescence to maximal fluorescence (Fv/Fm)] (P<0.05). In the latter 30 d, 0.5 μl/L NO2 showed a positive effect on the vitality of the seedlings, which was reflected by a recovery in the ratio of Fv/Fm and chlorophyll content, and obviously enhanced growth, SOD activity, ascorbate (AsA) content and glutathione reductase (GR) activity (P<0.05); 4.0 μl/L NO2 then showed a negative effect, indicated by significant reductions in chlorophyll content and the ratio of Fv/Fm, and inhibited growth (P<0.05). Conclusion: The results suggest adaptation of C. camphora seedlings to 60-d exposure to 0.1 and 0.5 μl/L NO2, but not to 60-d exposure to 4.0 μl/L NO2. C. camphora seedlings may protect themselves from injury by strengthening their antioxidant system in response to NO2-induced oxidative stress.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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