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Received: 2009-03-12

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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.10 P.753~760

10.1631/jzus.B0920072


Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China


Author(s):  Jian-ping LI, Hai-jian ZHOU, Lin YUAN, Ting HE, Song-hua HU

Affiliation(s):  Department of Veterinary Medicine, College of Animal Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   Songhua@zju.edu.cn

Key Words:  Staphylococcus aureus, Bovine mastitis, Antimicrobial resistance, Genetic diversity, Pulsed-field gel electrophoresis


Jian-ping LI, Hai-jian ZHOU, Lin YUAN, Ting HE, Song-hua HU. Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China[J]. Journal of Zhejiang University Science B, 2009, 10(10): 753~760.

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author="Jian-ping LI, Hai-jian ZHOU, Lin YUAN, Ting HE, Song-hua HU",
journal="Journal of Zhejiang University Science B",
volume="10",
number="10",
pages="753~760",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0920072"
}

%0 Journal Article
%T Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China
%A Jian-ping LI
%A Hai-jian ZHOU
%A Lin YUAN
%A Ting HE
%A Song-hua HU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 10
%P 753~760
%@ 1673-1581
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920072

TY - JOUR
T1 - Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China
A1 - Jian-ping LI
A1 - Hai-jian ZHOU
A1 - Lin YUAN
A1 - Ting HE
A1 - Song-hua HU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 10
SP - 753
EP - 760
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920072


Abstract: 
This study was conducted to determine genetic diversity and antimicrobial susceptibility profiles of Staphylococcus aureus recovered from bovine mastitis in Zhejiang Province, China. Out of 3178 quarter milk samples from 846 lactating cows, among which 459 cows (54.3%) were found HMT positive, 890 quarters (28%) were found having subclinical mastitis. From 75 representative S. aureus isolates, 16 distinct types were identified by pulsed-field gel electrophoresis (PFGE). Four major PFGE types (A, B, C, and D) accounted for 82.7% of all isolates, and type A (41.3%) was observed in multiple herds across the studied areas. Each region was found to have a predominant type: Hangzhou type A (64.1%), Ningbo type C (34.5%) and type B (23.1%), Jinhua type D (53.3%), and Taizhou type C (62.5%). Results of antimicrobial susceptibility tests showed that 90.7% of the isolates were resistant to at least one antimicrobial. Resistance to penicillin and ampicillin (77.3%), tetracycline (60.0%), or erythromycin (48.0%) was observed. The bacteria resistant to multiple antibiotics such as penicillin, ampicillin, tetracycline, and erythromycin were commonly found. The information obtained from this study is useful for designing specific control programs for bovine S. aureus mastitis in this region.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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