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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.11 P.860-866


A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO2 thin film

Author(s):  Xiao-lin ZONG, Chun-sheng WU, Xiao-ling WU, Yun-feng LU, Ping WANG

Affiliation(s):  Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   cnpwang@zju.edu.cn

Key Words:  DNA biosensor, Titanium dioxide (TiO2) thin film, Light addressable potentiometric sensor (LAPS), Silanization, Fluorescein label, Gene chip

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Xiao-lin ZONG, Chun-sheng WU, Xiao-ling WU, Yun-feng LU, Ping WANG. A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO2 thin film[J]. Journal of Zhejiang University Science B, 2009, 10(11): 860-866.

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author="Xiao-lin ZONG, Chun-sheng WU, Xiao-ling WU, Yun-feng LU, Ping WANG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO2 thin film
%A Xiao-lin ZONG
%A Chun-sheng WU
%A Xiao-ling WU
%A Yun-feng LU
%A Ping WANG
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 11
%P 860-866
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920090

T1 - A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO2 thin film
A1 - Xiao-lin ZONG
A1 - Chun-sheng WU
A1 - Xiao-ling WU
A1 - Yun-feng LU
A1 - Ping WANG
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 11
SP - 860
EP - 866
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0920090

titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane (APTS), fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that, with UV irradiation, the silanization level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample.

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