Full Text:   <3875>

CLC number: Q78

On-line Access: 

Received: 2009-07-14

Revision Accepted: 2009-11-03

Crosschecked: 2009-11-16

Cited: 12

Clicked: 6699

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.12 P.870-876

http://doi.org/10.1631/jzus.B0920204


Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette


Author(s):  Ming LIU, Jun YANG, Yun-qing CHENG, Li-jia AN

Affiliation(s):  Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, China; more

Corresponding email(s):   junyang@dlut.edu.cn, ljan.bio@gmail.com

Key Words:  Soybean, Glycine max (L.) Merrill, In planta ovary-drip transformation, Optimization, Style cutting, Minimal gus reporter gene cassette


Ming LIU, Jun YANG, Yun-qing CHENG, Li-jia AN. Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette[J]. Journal of Zhejiang University Science B, 2009, 10(12): 870-876.

@article{title="Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette",
author="Ming LIU, Jun YANG, Yun-qing CHENG, Li-jia AN",
journal="Journal of Zhejiang University Science B",
volume="10",
number="12",
pages="870-876",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0920204"
}

%0 Journal Article
%T Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette
%A Ming LIU
%A Jun YANG
%A Yun-qing CHENG
%A Li-jia AN
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 12
%P 870-876
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920204

TY - JOUR
T1 - Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette
A1 - Ming LIU
A1 - Jun YANG
A1 - Yun-qing CHENG
A1 - Li-jia AN
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 12
SP - 870
EP - 876
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920204


Abstract: 
soybean transformation by ovary-drip was improved by optimizing the length of the transformation pathway by cutting the styles. These modifications facilitated soybean transformation manipulation and improved transformation reproducibility and efficiency. Using a linear minimal gus gene cassette as the foreign DNA, a maximum transformation frequency of 11% was obtained in flowers of the soybean cultivar ‘Liaodou 14’ with their styles mostly removed, whereas removal of only the stigma, partial style cutting and partial ovary cutting gave transformation frequencies of 0%, 1%, and 2%, respectively. An average transformation frequency of 8.2% was obtained when 619 flowers from three soybean cultivars (‘Liaodou 14’, ‘Liaodou 13’, and ‘Tiefeng 29’) were transformed by this optimized method. Southern blotting analysis showed that the gus reporter gene (encoding β-glucuronidase) was stably inherited with a simple pattern. Reverse transcription-polymerase chain reaction (RT-PCR) and GUS staining confirmed the expression of the gus gene in transgenic plants.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Bechtold, N., Jaudeau, B., Jolivet, S., Maba, B., Vezon, D., Voisin, R., Pelletier, G., 2000. The maternal chromosome set is the target of the T-DNA in the in planta transformation of Arabidopsis thaliana. Genetics, 155(4): 1875-1887.

[2] Bent, A.F., 2000. Arabidopsis in planta transformation. Uses, mechanisms, and prospects for transformation of other species. Plant Physiol., 124(4):1540-1547.

[3] Bent, A.F., 2006. Arabidopsis thaliana floral dip transformation method. Methods Mol. Biol., 343:87-103.

[4] Chabaud, M., Ratet, P., de Sousa Araújo, S., Roldão Lopes Amaral Duque, A.S., Harrison, M., Barker, D.G., 2007. Agrobacterium tumefaciens-Mediated Transformation and in vitro Plant Regeneration of M. truncatula. Available from http://www.noble.org/MedicagoHandbook/ [Accessed on Oct. 8, 2009]

[5] Cheng, Y.Q., Yang, J., Xu, F.P., An, L.J., Liu, J.F., Chen, Z.W., 2009. Transient expression of minimum linear gene cassettes in onion epidermal cells via direct transformation. Appl. Biochem. Biotechnol., 159(3):739-749.

[6] Clough, S.J., Bent, A.F., 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J., 16(6):735-743.

[7] Desfeux, C., Clough, S.J., Bent, A.F., 2000. Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method. Plant Physiol., 123(3):895-904.

[8] Droste, A., Pasquali, G., Bodanese-Zanettini, H.M., 2002. Transgenic fertile plants of soybean [Glycine max (L.) Merrill] obtained from bombarded embryogenic tissue. Euphytica, 127(3):367-376.

[9] Gao, X.R., Wang, G.K., SU, Q., Wang, Y., AN, L.J., 2007. Phytase expression in transgenic soybeans: stable transformation with a vector-less construct. Biotechnol. Lett., 29(11):1781-1787.

[10] Hu, C.Y., Wang, L.Z., 1999. In planta soybean transformation technologies developed in China: procedure, confirmation and field performance. In Vitro Cell. Dev. Biol. Plant, 35(5):417-420.

[11] Jefferson, R.A., 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep., 5(4): 387-405.

[12] Liu, J.F., Su, Q., An, L.J., Yang, A.F., 2009. Transfer of a minimal linear marker-free and vector-free smGFP cassette into soybean via ovary-drip transformation. Biotechnol. Lett., 31(2):295-303.

[13] McCabe, D.E., Swain, W.F., Martinell, B.J., Christou, P., 1988. Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology, 6(8):923-926.

[14] Mello-Farias, P., Chaves, A., 2008. Advances in Agrobacterium-mediated plant transformation with emphasis on soybean. Sci. Agric. (Piracicaba, Braz.), 65(1):95-106.

[15] Murray, M.G., Thompson, W.F., 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids. Res., 8(19): 4321-4325.

[16] Paz, M.M., Shou, H., Guo, Z., Zhang, Z., Banerjee, A.K., Wang, K., 2004. Assessment of conditions affecting Agrobacterium-mediated soybean transformation using the cotyledonary node explant. Euphytica, 136(2): 167-179.

[17] Paz, M.M., Martinez, J.C., Kalvig, A.B., Fonger, T.M., Wang, K., 2006. Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation. Plant Cell Rep., 25(3):206-213.

[18] Sambrook, J., Tritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

[19] Santarém, E.R., Finer, J.J., 1999. Transformation of soybean (Glycine max (L.) Merrill) using proliferative embryogenic tissue maintained on semi-solid medium. In Vitro Cell. Dev. Biol. Plant, 35(6):451-455.

[20] Sato, S., Newell, C., Kolacz, K., Tredo, L., Finer, J.J., Hinchee, M., 1993. Stable transformation via particle bombardment in two different soybean regeneration systems. Plant Cell Rep., 12(7-8):408-413.

[21] Trieu, A.T., Burleigh, S.H., Kardailsky, I.V., Maldonado-Mendoza, I.E., Versaw, W.K., Blaylock, L.A., Shin, H., Chiou, T.J., Katagi, H., Dewbre, G.R., Weigel, D., Harrison, M.J., 2000. Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. Plant J., 22(6):531-541.

[22] Wu, W., Su, Q., Xia, X.Y., Wang, Y., Luan, Y.S., An, L.J., 2008. The Suaeda liaotungensis Kitag betaine aldehyde dehydrogenase gene improves salt tolerance of transgenic maize mediated with minimum linear length of DNA fragment. Euphytica, 159(1-2):17-25.

[23] Xue, R.G., Xie, H.F., Zhang, B., 2006. A multi-needle-assisted transformation of soybean cotyledonary node cells. Biotechnol. Lett., 28(19):1551-1557.

[24] Yang, A.F., Su, Q., An, L.J., Liu, J.F., Wu, W., Qiu, Z., 2009a. Detection of vector- and selectable marker-free transgenic maize with a linear GFP cassette transformation via the pollen-tube pathway. J. Biotechnol., 139(1):1-5.

[25] Yang, A.F., Su, Q., An, L., 2009b. Ovary-drip transformation: a simple method for directly generating vector- and marker-free transgenic maize (Zea mays L.) with a linear GFP cassette transformation. Planta, 229(4):793-801.

[26] Ye, G.N., Stone, D., Pang, S.Z., Creely, W., Gonzalez, K., Hinchee, M., 1999. Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation. Plant J., 19(3):249-257.

[27] Zhang, X., Henriques, R., Lin, S.S., Niu, Q.W., Chua, N.H., 2006. Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat. Protoc., 1(2):641-646.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE