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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.10 P.771-778

http://doi.org/10.1631/jzus.B1000111


Development of a large-scale isolation chamber system for the safe and humane care of medium-sized laboratory animals harboring infectious diseases


Author(s):  Xin Pan, Jian-cheng Qi, Ming Long, Hao Liang, Xiao Chen, Han Li, Guang-bo Li, Hao Zheng

Affiliation(s):  Department of Microbiology, the Second Military Medical University, Shanghai 200433, China

Corresponding email(s):   panxinpx@yahoo.com

Key Words:  Multi-function, Medium-sized animal, Isolation chamber, Biological safety


Xin Pan, Jian-cheng Qi, Ming Long, Hao Liang, Xiao Chen, Han Li, Guang-bo Li, Hao Zheng. Development of a large-scale isolation chamber system for the safe and humane care of medium-sized laboratory animals harboring infectious diseases[J]. Journal of Zhejiang University Science B, 2010, 11(10): 771-778.

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author="Xin Pan, Jian-cheng Qi, Ming Long, Hao Liang, Xiao Chen, Han Li, Guang-bo Li, Hao Zheng",
journal="Journal of Zhejiang University Science B",
volume="11",
number="10",
pages="771-778",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000111"
}

%0 Journal Article
%T Development of a large-scale isolation chamber system for the safe and humane care of medium-sized laboratory animals harboring infectious diseases
%A Xin Pan
%A Jian-cheng Qi
%A Ming Long
%A Hao Liang
%A Xiao Chen
%A Han Li
%A Guang-bo Li
%A Hao Zheng
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 10
%P 771-778
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000111

TY - JOUR
T1 - Development of a large-scale isolation chamber system for the safe and humane care of medium-sized laboratory animals harboring infectious diseases
A1 - Xin Pan
A1 - Jian-cheng Qi
A1 - Ming Long
A1 - Hao Liang
A1 - Xiao Chen
A1 - Han Li
A1 - Guang-bo Li
A1 - Hao Zheng
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 10
SP - 771
EP - 778
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000111


Abstract: 
The close phylogenetic relationship between humans and non-human primates makes non-human primates an irreplaceable model for the study of human infectious diseases. In this study, we describe the development of a large-scale automatic multi-functional isolation chamber for use with medium-sized laboratory animals carrying infectious diseases. The isolation chamber, including the transfer chain, disinfection chain, negative air pressure isolation system, animal welfare system, and the automated system, is designed to meet all biological safety standards. To create an internal chamber environment that is completely isolated from the exterior, variable frequency drive blowers are used in the air-intake and air-exhaust system, precisely controlling the filtered air flow and providing an air-barrier protection. A double door transfer port is used to transfer material between the interior of the isolation chamber and the outside. A peracetic acid sterilizer and its associated pipeline allow for complete disinfection of the isolation chamber. All of the isolation chamber parameters can be automatically controlled by a programmable computerized menu, allowing for work with different animals in different-sized cages depending on the research project. The large-scale multi-functional isolation chamber provides a useful and safe system for working with infectious medium-sized laboratory animals in high-level bio-safety laboratories.

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