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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.1 P.1-11

http://doi.org/10.1631/jzus.B1000196


Whole genome amplification in preimplantation genetic diagnosis


Author(s):  Ying-ming Zheng, Ning Wang, Lei Li, Fan Jin

Affiliation(s):  Department of Reproductive Endocrinology, Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China

Corresponding email(s):   jinfan@zju.edu.cn

Key Words:  Whole genome amplification, Multiple displacement amplification, Primer extension preamplification, Degenerate oligonucleotide primed-polymerase chain reaction, Preimplantation genetic diagnosis


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Ying-ming Zheng, Ning Wang, Lei Li, Fan Jin. Whole genome amplification in preimplantation genetic diagnosis[J]. Journal of Zhejiang University Science B, 2011, 12(1): 1-11.

@article{title="Whole genome amplification in preimplantation genetic diagnosis",
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pages="1-11",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000196"
}

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%DOI 10.1631/jzus.B1000196

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T1 - Whole genome amplification in preimplantation genetic diagnosis
A1 - Ying-ming Zheng
A1 - Ning Wang
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1000196


Abstract: 
preimplantation genetic diagnosis (PGD) refers to a procedure for genetically analyzing embryos prior to implantation, improving the chance of conception for patients at high risk of transmitting specific inherited disorders. This method has been widely used for a large number of genetic disorders since the first successful application in the early 1990s. Polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) are the two main methods in PGD, but there are some inevitable shortcomings limiting the scope of genetic diagnosis. Fortunately, different whole genome amplification (WGA) techniques have been developed to overcome these problems. Sufficient DNA can be amplified and multiple tasks which need abundant DNA can be performed. Moreover, WGA products can be analyzed as a template for multi-loci and multi-gene during the subsequent DNA analysis. In this review, we will focus on the currently available WGA techniques and their applications, as well as the new technical trends from WGA products.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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