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CLC number: R779.6

On-line Access: 2011-06-07

Received: 2010-06-02

Revision Accepted: 2011-02-28

Crosschecked: 2011-05-14

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.6 P.492-498

http://doi.org/10.1631/jzus.B1000199


Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy


Author(s):  Ting-jun Fan, Jun Zhao, Xiu-zhong Hu, Xi-ya Ma, Wen-bo Zhang, Chao-zhong Yang

Affiliation(s):  Key Laboratory for Corneal Tissue Engineering, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China, Qingdao Dongfang Eye Hospital, Qingdao 266110, China

Corresponding email(s):   tjfan@ouc.edu.cn

Key Words:  Tissue-engineered human corneal endothelium, New Zealand white rabbits, Penetrating keratoplasty, Primary corneal endotheliopathy, Transparency


Ting-jun Fan, Jun Zhao, Xiu-zhong Hu, Xi-ya Ma, Wen-bo Zhang, Chao-zhong Yang. Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy[J]. Journal of Zhejiang University Science B, 2011, 12(6): 492-498.

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author="Ting-jun Fan, Jun Zhao, Xiu-zhong Hu, Xi-ya Ma, Wen-bo Zhang, Chao-zhong Yang",
journal="Journal of Zhejiang University Science B",
volume="12",
number="6",
pages="492-498",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000199"
}

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%T Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy
%A Ting-jun Fan
%A Jun Zhao
%A Xiu-zhong Hu
%A Xi-ya Ma
%A Wen-bo Zhang
%A Chao-zhong Yang
%J Journal of Zhejiang University SCIENCE B
%V 12
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%P 492-498
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000199

TY - JOUR
T1 - Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy
A1 - Ting-jun Fan
A1 - Jun Zhao
A1 - Xiu-zhong Hu
A1 - Xi-ya Ma
A1 - Wen-bo Zhang
A1 - Chao-zhong Yang
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 6
SP - 492
EP - 498
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1000199


Abstract: 
To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP), TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mcHCECs) and modified denuded amniotic membranes (mdAMs) were transplanted into PCEP models of new Zealand white rabbits using penetrating keratoplasty. The TE-HCEs were examined using diverse techniques including slit-lamp biomicroscopy observation and pachymeter and tonometer measurements in vivo, and fluorescent microscopy, alizarin red staining, paraffin sectioning, scanning and transmission electron microscopy observations in vitro. The corneas of transplanted eyes maintained transparency for as long as 200 d without obvious edema or immune rejection. The corneal thickness of transplanted eyes decreased gradually after transplanting, reaching almost the thickness of normal eyes after 156 d, while the TE-HCE non-transplanted eyes were turbid and showed obvious corneal edema. The polygonal corneal endothelial cells in the transplanted area originated from the TE-HCE transplant. An intact monolayer corneal endothelium had been reconstructed with the morphology, cell density and structure similar to those of normal rabbit corneal endothelium. In conclusion, the transplanted TE-HCE can reconstruct the integrality of corneal endothelium and restore corneal transparency and thickness in PCEP rabbits. The TE-HCE functions normally as an endothelial barrier and pump and promises to be an equivalent of HCE for clinical therapy of human PCEP.

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