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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.6 P.436-447


Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

Author(s):  Yuan-fei Zhou, Xian-yin Zhang, Qing-zhong Xue

Affiliation(s):  Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   xueqingzhong@hotmail.com

Key Words:  Super hybrid rice, pms1(t), Photoperiod and thermo-sensitive male sterile gene, Genetic mappingThe online version of this article contains supplementary materials

Yuan-fei Zhou, Xian-yin Zhang, Qing-zhong Xue. Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice[J]. Journal of Zhejiang University Science B, 2011, 12(6): 436-447.

@article{title="Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice",
author="Yuan-fei Zhou, Xian-yin Zhang, Qing-zhong Xue",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice
%A Yuan-fei Zhou
%A Xian-yin Zhang
%A Qing-zhong Xue
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 6
%P 436-447
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000306

T1 - Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice
A1 - Yuan-fei Zhou
A1 - Xian-yin Zhang
A1 - Qing-zhong Xue
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 6
SP - 436
EP - 447
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000306

Pei′ai64S, an indica sterile variety with photoperiod and thermo-sensitive genic male sterile (PTGMS) genes, has been widely exploited for commercial seed production for “two-line” hybrid rice in China. One PTGMS gene from Pei′ai64S, pms1(t), was mapped by a strategy of bulked-extreme and recessive-class approach with simple sequence repeat (SSR) and insert and deletion (In-Del) markers. Using linkage analysis for the F2 mapping population consisting of 320 completely male sterile individuals derived from a cross between Pei′ai64S and 93-11 (indica restorer) lines, the pms1(t) gene was delimited to the region between the RM21242 (0.2 cM) and YF11 (0.2 cM) markers on the short arm of chromosome 7. The interval containing the pms1(t) locus, which was co-segregated with RM6776, is a 101.1 kb region based on the Nipponbare rice genome. Fourteen predicted loci were found in this region by the Institute for Genomic Research (TIGR) Genomic Annotation. Based on the function of the locus LOC_Os07g12130 by bioinformatics analysis, it is predicted to encode a protein containing a Myb-like DNA-binding domain, and may process the transcript with thermosensory response. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the mRNA levels of LOC_Os07g12130 were altered in different photoperiod and temperature treatments. Thus, the LOC_Os07g12130 locus is the most likely candidate gene for pms1(t). These results may facilitate not only using the molecular marker assisted selection of PTGMS genes, but also cloning of the pms1(t) gene itself.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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