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CLC number: R45

On-line Access: 2011-04-06

Received: 2010-11-08

Revision Accepted: 2011-03-04

Crosschecked: 2011-03-16

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.4 P.264-272


Efficacy of a novel endotoxin adsorber polyvinylidene fluoride fiber immobilized with L-serine ligand on septic pigs

Author(s):  Jian-ping Gao, Man Huang, Ning Li, Peng-fei Wang, Huan-lin Chen, Qiu-ping Xu

Affiliation(s):  Critical Care Department, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China, Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   xu_qp@yahoo.cn

Key Words:  Sepsis, Endotoxin, Adsorption, Polyvinylidene fluoride matrix immobilized with L-serine ligand (PVDF-Ser), Hemoperfusion, Pig

Jian-ping Gao, Man Huang, Ning Li, Peng-fei Wang, Huan-lin Chen, Qiu-ping Xu. Efficacy of a novel endotoxin adsorber polyvinylidene fluoride fiber immobilized with L-serine ligand on septic pigs[J]. Journal of Zhejiang University Science B, 2011, 12(4): 264-272.

@article{title="Efficacy of a novel endotoxin adsorber polyvinylidene fluoride fiber immobilized with L-serine ligand on septic pigs",
author="Jian-ping Gao, Man Huang, Ning Li, Peng-fei Wang, Huan-lin Chen, Qiu-ping Xu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Efficacy of a novel endotoxin adsorber polyvinylidene fluoride fiber immobilized with L-serine ligand on septic pigs
%A Jian-ping Gao
%A Man Huang
%A Ning Li
%A Peng-fei Wang
%A Huan-lin Chen
%A Qiu-ping Xu
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 4
%P 264-272
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000389

T1 - Efficacy of a novel endotoxin adsorber polyvinylidene fluoride fiber immobilized with L-serine ligand on septic pigs
A1 - Jian-ping Gao
A1 - Man Huang
A1 - Ning Li
A1 - Peng-fei Wang
A1 - Huan-lin Chen
A1 - Qiu-ping Xu
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 4
SP - 264
EP - 272
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000389

A novel adsorber, polyvinylidene fluoride matrix immobilized with L-serine ligand (PVDF-Ser), was developed in the present study to evaluate its safety and therapeutic efficacy in septic pigs by extracorporeal hemoperfusion. endotoxin adsorption efficiency (EAE) of the adsorber was firstly measured in vitro. The biocompatibility and hemodynamic changes during extracorporeal circulation were then evaluated. One half of 16 pigs receiving lipopolysaccharide (Escherichia coli O111:B4, 5 μg/kg) intravenously in 1 h were consecutively treated by hemoperfusion with the new adsorber for 2 h. The changes of circulating endotoxin and certain cytokines and respiratory function were analyzed. The 72 h-survival rate was assessed eventually. EAE reached 46.3% (100 EU/ml in 80 ml calf serum) after 2 h-circulation. No deleterious effect was observed within the process. The plasma endotoxin, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels were decreased during the hemoperfusion. Arterial oxygenation was also improved during and after the process. Furthermore, the survival time was significantly extended (>72 h vs. 47.5 h for median survival time). The novel product PVDF-Ser could adsorb endotoxin with high safety and efficacy. Early use of extracorporeal hemoperfusion with the new adsorber could reduce the levels of circulating endotoxin, IL-6, and TNF-α, besides improve respiratory function and consequent 72 h-survival rate of the septic pigs. endotoxin removal strategy with blood purification using the new adsorber renders a potential promising future in sepsis therapy.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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