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On-line Access: 2010-08-02

Received: 2010-06-13

Revision Accepted: 2010-07-05

Crosschecked: 2010-07-08

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.8 P.608-617

10.1631/jzus.B1001007


Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways


Author(s):  Feng Gao, Xin-yang Hu, Xiao-jie Xie, Qi-yuan Xu, Ya-ping Wang, Xian-bao Liu, Mei-xiang Xiang, Yong Sun, Jian-an Wang

Affiliation(s):  Department of Cardiology; more

Corresponding email(s):   wang_jian_an@tom.com

Key Words:  Heat shock protein, Apoptosis, Stem cell, Hypoxia, Phosphoinositide-3-kinase/protein kinase B (PI3K/Akt), Extracellular-signal-regulate kinase (ERK)


Feng Gao, Xin-yang Hu, Xiao-jie Xie, Qi-yuan Xu, Ya-ping Wang, Xian-bao Liu, Mei-xiang Xiang, Yong Sun, Jian-an Wang. Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways[J]. Journal of Zhejiang University Science B, 2010, 11(8): 608-617.

@article{title="Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways",
author="Feng Gao, Xin-yang Hu, Xiao-jie Xie, Qi-yuan Xu, Ya-ping Wang, Xian-bao Liu, Mei-xiang Xiang, Yong Sun, Jian-an Wang",
journal="Journal of Zhejiang University Science B",
volume="11",
number="8",
pages="608-617",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1001007"
}

%0 Journal Article
%T Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways
%A Feng Gao
%A Xin-yang Hu
%A Xiao-jie Xie
%A Qi-yuan Xu
%A Ya-ping Wang
%A Xian-bao Liu
%A Mei-xiang Xiang
%A Yong Sun
%A Jian-an Wang
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 8
%P 608-617
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1001007

TY - JOUR
T1 - Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways
A1 - Feng Gao
A1 - Xin-yang Hu
A1 - Xiao-jie Xie
A1 - Qi-yuan Xu
A1 - Ya-ping Wang
A1 - Xian-bao Liu
A1 - Mei-xiang Xiang
A1 - Yong Sun
A1 - Jian-an Wang
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 8
SP - 608
EP - 617
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1001007


Abstract: 
Mesenchymal stem cell (MSC) transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium, but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment. The aim of this study is to explore the cytoprotection of heat shock protein 90 (Hsp90) against hypoxia and serum deprivation-induced apoptosis and the possible mechanisms in rat MSCs. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. apoptosis was assessed by Hoechst 33258 nuclear staining and flow cytometric analysis with annexin V/PI staining. The gene expression of Toll-like receptor-4 (TLR-4) and V-erb-b2 erythroblastic leukemia viral oncogene homolog 2 (ErbB2) was detected by real-time polymerase chain reaction (PCR). The protein levels of cleaved caspase-3, Bcl-2, Bcl-xL, Bax, total-ERK, phospho-ERK, total-Akt, phospho-Akt, and Hsp90 were detected by Western blot. The production of nitric oxide was measured by spectrophotometric assay. Hsp90 improves MSC viability and protects MSCs against apoptosis induced by serum deprivation and hypoxia. The protective role of Hsp90 not only elevates Bcl-2/Bax and Bcl-xL/Bax expression and attenuates cleaved caspase-3 expression via down-regulating membrane TLR-4 and ErbB2 receptors and then activating their downstream PI3K/Akt and ERK1/2 pathways, but also enhances the paracrine effect of MSCs. These findings demonstrated a novel and effective treatment strategy against MSC apoptosis in cell transplantation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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