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On-line Access: 2011-08-02

Received: 2011-06-29

Revision Accepted: 2011-07-05

Crosschecked: 2011-06-29

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.8 P.644-651


Tongxinluo promotes mesenchymal stem cell tube formation in vitro

Author(s):  Xin-yang Hu, Wen-xia Wang, Min-jia Yu, Xian-bao Liu, Rong-rong Wu, Feng Gao, Xin Huang, Jiang Cao, Xiao-jie Xie, Jian-an Wang

Affiliation(s):  Cardiovascular Key Lab of Zhejiang Province, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China

Corresponding email(s):   Wang_jian_an@tom.com

Key Words:  Bone marrow mesenchymal stem cells, Tongxinluo, Angiogenesis, Migration, Tube formation

Xin-yang Hu, Wen-xia Wang, Min-jia Yu, Xian-bao Liu, Rong-rong Wu, Feng Gao, Xin Huang, Jiang Cao, Xiao-jie Xie, Jian-an Wang. Tongxinluo promotes mesenchymal stem cell tube formation in vitro[J]. Journal of Zhejiang University Science B, 2011, 12(8): 644-651.

@article{title="Tongxinluo promotes mesenchymal stem cell tube formation in vitro",
author="Xin-yang Hu, Wen-xia Wang, Min-jia Yu, Xian-bao Liu, Rong-rong Wu, Feng Gao, Xin Huang, Jiang Cao, Xiao-jie Xie, Jian-an Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Tongxinluo promotes mesenchymal stem cell tube formation in vitro
%A Xin-yang Hu
%A Wen-xia Wang
%A Min-jia Yu
%A Xian-bao Liu
%A Rong-rong Wu
%A Feng Gao
%A Xin Huang
%A Jiang Cao
%A Xiao-jie Xie
%A Jian-an Wang
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 8
%P 644-651
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1101005

T1 - Tongxinluo promotes mesenchymal stem cell tube formation in vitro
A1 - Xin-yang Hu
A1 - Wen-xia Wang
A1 - Min-jia Yu
A1 - Xian-bao Liu
A1 - Rong-rong Wu
A1 - Feng Gao
A1 - Xin Huang
A1 - Jiang Cao
A1 - Xiao-jie Xie
A1 - Jian-an Wang
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 8
SP - 644
EP - 651
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1101005

Objective: To study whether tongxinluo (TXL) can induce angiogenesis in bone marrow mesenchymal stem cells (MSCs), and to investigate the underlying mechanism. Methods: Bone marrow MSCs were obtained from male Sprague-Dawley rats. We established an angiogenesis model in vitro via matrigel experiment. MSCs were seeded on matrigel coated 24-well plates, and treated by TXL 50 and 100 mg/L. After 24 h, we observed the tube formations of MSCs in the matrigel. Cell migration ability was examined by wound scratch test and transwell assay. Expressions of vascular endothelial growth factor (VEGF), fetal liver kinase-1 (Flk-1), matrix metalloproteinase-2 (MMP-2), MMP-9, and tissue inhibitor of metalloproteinase-2 (TIMP-2) were analyzed at the protein level by Western blot. Gelatin zymography assay was applied to investigating the MSC paracrine abilities of pro-MMP-2 and activated MMP-2. Results: TXL promoted MSC tube formation in matrigel. The ratio of TXL 100 mg/L treated-MSC tubular length was increased 3.04-fold compared to the control group (P<0.05). Scratch test and transwell assay showed that TXL could improve the cell migration ability of MSCs. Western blot experiments showed that TXL promoted MSC synthesis of MMP-2, but it had no influence on the expressions of MMP-9 and TIMP-2. This effect was confirmed by gelatin zymography assay, which showed that TXL increased MSC secretion of pro-MMP-2 and activated MMP-2. VEGF expression of TXL treated-MSCs was increased compared to the control group. The expression of Flk-1 was not different among the groups. Conclusions: This study demonstrates that TXL can promote the tube formation of MSCs, and the underlying mechanisms are associated with increased migration ability of MSCs and the up-regulation of MMP-2 and VEGF expressions.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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