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CLC number: Q945.78

On-line Access: 2012-12-07

Received: 2012-03-21

Revision Accepted: 2012-07-29

Crosschecked: 2012-10-31

Cited: 2

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.12 P.1006-1014


An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H2S emission

Author(s):  Ya-wei Li, Ze-hua Gong, Yao Mu, Yi-xian Zhang, Zeng-jie Qiao, Li-ping Zhang, Zhu-ping Jin, Hua Li, Yan-xi Pei

Affiliation(s):  School of Life Science, Shanxi University, Taiyuan 030006, China; more

Corresponding email(s):   peiyanxi@sxu.edu.cn

Key Words:  AtCSR, H2S gas-transmitter, Glutathione, Cadmium stress sensitivity

Ya-wei Li, Ze-hua Gong, Yao Mu, Yi-xian Zhang, Zeng-jie Qiao, Li-ping Zhang, Zhu-ping Jin, Hua Li, Yan-xi Pei. An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H2S emission[J]. Journal of Zhejiang University Science B, 2012, 13(12): 1006-1014.

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author="Ya-wei Li, Ze-hua Gong, Yao Mu, Yi-xian Zhang, Zeng-jie Qiao, Li-ping Zhang, Zhu-ping Jin, Hua Li, Yan-xi Pei",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H2S emission
%A Ya-wei Li
%A Ze-hua Gong
%A Yao Mu
%A Yi-xian Zhang
%A Zeng-jie Qiao
%A Li-ping Zhang
%A Zhu-ping Jin
%A Hua Li
%A Yan-xi Pei
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%V 13
%N 12
%P 1006-1014
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200089

T1 - An Arabidopsis mutant atcsr-2 exhibits high cadmium stress sensitivity involved in the restriction of H2S emission
A1 - Ya-wei Li
A1 - Ze-hua Gong
A1 - Yao Mu
A1 - Yi-xian Zhang
A1 - Zeng-jie Qiao
A1 - Li-ping Zhang
A1 - Zhu-ping Jin
A1 - Hua Li
A1 - Yan-xi Pei
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 12
SP - 1006
EP - 1014
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200089

The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production. In our studies, we found that AtCSR was associated with cadmium (Cd)-sensitive response in Arabidopsis. Our results show that AtCSR expression was triggered by Cd-stress in wild type Arabidopsis. The expression of some genes responsible for Cd2+ transportation into vacuoles was induced, and the expression of the iron-regulated transporter 1 (IRT1) related to Cd2+ absorption from the environment was not induced in wild type with Cd2+ treatment. The expression of Cd-transportation related genes was not in response to Cd-stress, whereas IRT expression increased dramatically in atcsr-2 with Cd2+ treatment. The expression of glutathione 1 (GSH1) was consistent with GSH being much lower in atcsr-2 in comparison with the wild type with Cd2+ treatment. Additionally, malondialdehyde (MDA), hydrogen peroxide, and Cd2+ contents, and activities of some antioxidative enzymes, differed between the wild type and atcsr-2. Hydrogen sulfide (H2S) has been confirmed as the third gas-transmitter over recent years. The findings revealed that the expression pattern of H2S-releasing related genes and that of Cd-induced chelation and transportation genes matched well in the wild type and atcsr-2, and H2S could regulate the expression of the Cd-induced genes and alleviate Cd-triggered toxicity. Finally, one possible suggestion was given: down-regulation of atcsr-2, depending on h2S gas-transmitter not only weakened Cd2+ chelation, but also reduced Cd2+ transportation into vacuoles, as well as enhancing the Cd2+ assimilation, thus rendering atcsr-2 mutant sensitive to Cd-stress.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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