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CLC number: Q815

On-line Access: 2013-04-03

Received: 2012-05-30

Revision Accepted: 2012-10-31

Crosschecked: 2013-03-07

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.4 P.346-354

10.1631/jzus.B1200153


Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology


Author(s):  Hua Chen, Mian-bin Wu, Zheng-jie Chen, Ming-lu Wang, Jian-ping Lin, Li-rong Yang

Affiliation(s):  Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   chenhua0563@yahoo.com.cn, wumb@zju.edu.cn, linjp@zju.edu.cn

Key Words:  24-membered ring macrolide, Enhancing production, Response surface methodology, Faeces bombycis, Marine bacterium


Hua Chen, Mian-bin Wu, Zheng-jie Chen, Ming-lu Wang, Jian-ping Lin, Li-rong Yang. Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology[J]. Journal of Zhejiang University Science B, 2013, 14(4): 346-354.

@article{title="Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology",
author="Hua Chen, Mian-bin Wu, Zheng-jie Chen, Ming-lu Wang, Jian-ping Lin, Li-rong Yang",
journal="Journal of Zhejiang University Science B",
volume="14",
number="4",
pages="346-354",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200153"
}

%0 Journal Article
%T Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology
%A Hua Chen
%A Mian-bin Wu
%A Zheng-jie Chen
%A Ming-lu Wang
%A Jian-ping Lin
%A Li-rong Yang
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 4
%P 346-354
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200153

TY - JOUR
T1 - Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology
A1 - Hua Chen
A1 - Mian-bin Wu
A1 - Zheng-jie Chen
A1 - Ming-lu Wang
A1 - Jian-ping Lin
A1 - Li-rong Yang
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 4
SP - 346
EP - 354
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200153


Abstract: 
A 24-membered ring macrolide compound, macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity. In this study, macrolactin A was produced by a marine bacterium, which was identified as Bacillus subtilis by 16S ribosomal RNA (rRNA) sequence analysis. Electrospray ionization mass spectrometry (ESI/MS) and nuclear magnetic resonance (NMR) spectroscopy analyses were used to characterize this compound. To improve the production, response surface methodology (RSM) involving Box-Behnken design (BBD) was employed. Faeces bombycis, the main by-product in sericulture, was used as a nitrogen source in fermentation. The interactions between three significant factors, F. bombycis, soluble starch, and (NH4)2SO4 were investigated. A quadratic model was constructed to fit the production and the factors. Optimum medium composition was obtained by analysis of the model. When cultivated in the optimum medium, the production of macrolactin A was increased to 851 mg/L, 2.7 times as compared to the original. This study is also useful to find another way in utilizing F. bombycis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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