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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.6 P.511-517

http://doi.org/10.1631/jzus.B1200195


Activation of Akt and cardioprotection against reperfusion injury are maximal with only five minutes of sevoflurane postconditioning in isolated rat hearts


Author(s):  Yuan-yuan Yao, Man-hua Zhu, Feng-jiang Zhang, Chuan-yun Wen, Lei-lei Ma, Wen-na Wang, Can-can Wang, Xian-bao Liu, Li-na Yu, Ling-bo Qian, Jian-an Wang, Min Yan

Affiliation(s):  Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   yanminnina@hotmail.com

Key Words:  Sevoflurane postconditioning, Ischemia/reperfusion (I/R) injury, Cardioprotection, Duration of administration, Akt


Yuan-yuan Yao, Man-hua Zhu, Feng-jiang Zhang, Chuan-yun Wen, Lei-lei Ma, Wen-na Wang, Can-can Wang, Xian-bao Liu, Li-na Yu, Ling-bo Qian, Jian-an Wang, Min Yan. Activation of Akt and cardioprotection against reperfusion injury are maximal with only five minutes of sevoflurane postconditioning in isolated rat hearts[J]. Journal of Zhejiang University Science B, 2013, 14(6): 511-517.

@article{title="Activation of Akt and cardioprotection against reperfusion injury are maximal with only five minutes of sevoflurane postconditioning in isolated rat hearts",
author="Yuan-yuan Yao, Man-hua Zhu, Feng-jiang Zhang, Chuan-yun Wen, Lei-lei Ma, Wen-na Wang, Can-can Wang, Xian-bao Liu, Li-na Yu, Ling-bo Qian, Jian-an Wang, Min Yan",
journal="Journal of Zhejiang University Science B",
volume="14",
number="6",
pages="511-517",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200195"
}

%0 Journal Article
%T Activation of Akt and cardioprotection against reperfusion injury are maximal with only five minutes of sevoflurane postconditioning in isolated rat hearts
%A Yuan-yuan Yao
%A Man-hua Zhu
%A Feng-jiang Zhang
%A Chuan-yun Wen
%A Lei-lei Ma
%A Wen-na Wang
%A Can-can Wang
%A Xian-bao Liu
%A Li-na Yu
%A Ling-bo Qian
%A Jian-an Wang
%A Min Yan
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 6
%P 511-517
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200195

TY - JOUR
T1 - Activation of Akt and cardioprotection against reperfusion injury are maximal with only five minutes of sevoflurane postconditioning in isolated rat hearts
A1 - Yuan-yuan Yao
A1 - Man-hua Zhu
A1 - Feng-jiang Zhang
A1 - Chuan-yun Wen
A1 - Lei-lei Ma
A1 - Wen-na Wang
A1 - Can-can Wang
A1 - Xian-bao Liu
A1 - Li-na Yu
A1 - Ling-bo Qian
A1 - Jian-an Wang
A1 - Min Yan
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 6
SP - 511
EP - 517
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200195


Abstract: 
It had been proved that administration of sevoflurane for the first two minutes of reperfusion effectively protects the heart against reperfusion injury in rats in vivo. Our aim was to investigate the duration of effective sevoflurane administration and its underlying mechanism in isolated rat hearts exposed to global ischemia/reperfusion (I/R) injury. Adult male Sprague-Dawley rats were randomly divided into six groups (n=12): a sham-operation group, an I/R group, and four sevoflurane postconditioning groups (S2, S5, S10, and S15). In the S2, S5, S10, and S15 groups, the duration times of sevoflurane administration were 2, 5, 10, and 15 min after the onset of reperfusion, respectively. The isolated rat hearts were mounted on the Langendorff system, and after a period of equilibrium were subjected to 40 min global ischemia and 120 min reperfusion. Left ventricular (LV) hemodynamic parameters were monitored throughout each experiment and the data at 30 min of equilibrium and 30, 60, 90, and 120 min of reperfusion were analyzed. Myocardial infarct size at the end of reperfusion (n=7 in each group) and the expression of myocardial phosphorylated akt (p-akt) after 15-min reperfusion were determined in a duplicate set of six groups of rat hearts (n=5 in each group). Compared with the I/R group, the S5, S10, and S15 groups had significantly improved left ventricular end-diastolic pressure (LVEDP), left ventricular developed pressure (LVDP), and the maximal rate of rise or fall of the LV pressure (±dP/dtmax), and decreased myocardial infarct size (P<0.05), but not the S2 group. After 15 min of reperfusion, the expression of p-akt was markedly up-regulated in the S5, S10, and S15 groups compared with that in the I/R group (P<0.05), but not in the S2 group. sevoflurane postconditioning for 5 min was sufficient to activate akt and exert maximal cardioprotection against I/R injury in isolated rat hearts.

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