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On-line Access: 2013-07-04

Received: 2012-08-23

Revision Accepted: 2012-11-15

Crosschecked: 2013-06-24

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.7 P.570-577

10.1631/jzus.B1200227


Diversity arrays technology (DArT) for studying the genetic polymorphism of flue-cured tobacco (Nicotiana tabacum)


Author(s):  Xiu-ping Lu, Bing-guang Xiao, Yong-ping Li, Yi-jie Gui, Yu Wang, Long-jiang Fan

Affiliation(s):  Department of Agronomy, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   xiaobg@263.net, fanlj@zju.edu.cn

Key Words:  Nicotiana tabacum, Diversity arrays technology (DArT), Genetic diversity, Flue-cured tobacco The online version of this article contains supplementary materialssupplementary materials


Xiu-ping Lu, Bing-guang Xiao, Yong-ping Li, Yi-jie Gui, Yu Wang, Long-jiang Fan. Diversity arrays technology (DArT) for studying the genetic polymorphism of flue-cured tobacco (Nicotiana tabacum)[J]. Journal of Zhejiang University Science B, 2013, 14(7): 570-577.

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author="Xiu-ping Lu, Bing-guang Xiao, Yong-ping Li, Yi-jie Gui, Yu Wang, Long-jiang Fan",
journal="Journal of Zhejiang University Science B",
volume="14",
number="7",
pages="570-577",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200227"
}

%0 Journal Article
%T Diversity arrays technology (DArT) for studying the genetic polymorphism of flue-cured tobacco (Nicotiana tabacum)
%A Xiu-ping Lu
%A Bing-guang Xiao
%A Yong-ping Li
%A Yi-jie Gui
%A Yu Wang
%A Long-jiang Fan
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 7
%P 570-577
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200227

TY - JOUR
T1 - Diversity arrays technology (DArT) for studying the genetic polymorphism of flue-cured tobacco (Nicotiana tabacum)
A1 - Xiu-ping Lu
A1 - Bing-guang Xiao
A1 - Yong-ping Li
A1 - Yi-jie Gui
A1 - Yu Wang
A1 - Long-jiang Fan
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 7
SP - 570
EP - 577
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200227


Abstract: 
diversity arrays technology (DArT) is a microarray-based marker system that achieves high throughput by reducing the complexity of the genome. A DArT chip has recently been developed for tobacco. In this study, we genotyped 267 flue-cured cultivars/landraces, including 121 Chinese accessions over five decades from widespread geographic regions in China, 103 from the Americas, and 43 other foreign cultivars, using the newly developed chip. Three hundred and thirty polymorphic DArT makers were selected and used for a phylogenetic analysis, which suggested that the 267 accessions could be classified into two subgroups, which could each be further divided into 2‒4 sections. Eight elite cultivars, which account for 83% of the area of Chinese tobacco production, were all found in one subgroup. Two high-quality cultivars, HHDJY and Cuibi1, were grouped together in one section, while six other high-yield cultivars were grouped into another section. The 330 DArT marker clones were sequenced and close to 95% of them are within non-repetitive regions. Finally, the implications of this study for Chinese flue-cured tobacco breeding and production programs were discussed.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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