Full Text:   <2153>

CLC number: S432.1

On-line Access: 2013-04-03

Received: 2012-09-06

Revision Accepted: 2012-11-09

Crosschecked: 2013-03-15

Cited: 4

Clicked: 3796

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.4 P.336-345


Ultrastructure and phylogeny of Ustilago coicis

Author(s):  Jing-ze Zhang, Pei-gang Guan, Gang Tao, Mohammad Reza Ojaghian, Kevin David Hyde

Affiliation(s):  Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   jzzhang@zju.edu.cn

Key Words:  Fungus-host interaction, Molecular phylogenetics, Smut, Teliospore wall development

Jing-ze Zhang, Pei-gang Guan, Gang Tao, Mohammad Reza Ojaghian, Kevin David Hyde. Ultrastructure and phylogeny of Ustilago coicis[J]. Journal of Zhejiang University Science B, 2013, 14(4): 336-345.

@article{title="Ultrastructure and phylogeny of Ustilago coicis",
author="Jing-ze Zhang, Pei-gang Guan, Gang Tao, Mohammad Reza Ojaghian, Kevin David Hyde",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Ultrastructure and phylogeny of Ustilago coicis
%A Jing-ze Zhang
%A Pei-gang Guan
%A Gang Tao
%A Mohammad Reza Ojaghian
%A Kevin David Hyde
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 4
%P 336-345
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200239

T1 - Ultrastructure and phylogeny of Ustilago coicis
A1 - Jing-ze Zhang
A1 - Pei-gang Guan
A1 - Gang Tao
A1 - Mohammad Reza Ojaghian
A1 - Kevin David Hyde
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 4
SP - 336
EP - 345
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200239

Ustilago coicis causes serious smut on Coix lacryma-jobi in Dayang Town, Jinyun County, Zhejiang Province of China. In this paper, ultrastructural assessments on fungus-host interactions and teliospore development are presented, and molecular phylogenetic analyses have been done to elucidate the phylogenetic placement of the taxon. Hyphal growth within infected tissues was both intracellular and intercellular and on the surface of fungus-host interaction, and the fungal cell wall and the invaginated host plasma membrane were separated by a sheath comprising two distinct layers between the fungal cell wall and the invaginated host plasma membrane. Ornamentation development of teliospore walls was unique as they appeared to be originated from the exosporium. In addition, internal transcribed spacer (ITS) and large subunit (LSU) sequence data showed that U. coicis is closely related to Ustilago trichophora which infects grass species of the genus Echinochloa (Poaceae).

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Bauer, R., Oberwinkler, F., Vánky, K., 1997. Ultrastructural markers and systematics in smut fungi and allied taxa. Can. J. Bot., 75(8):1273-1314.

[2]Begerow, D., Bauer, R., Oberwinkler, F., 1997. Phylogenetic studies on nuclear large subunit ribosomal DNA sequences of smut fungi and related taxa. Can. J. Bot., 75(12):2045-2056.

[3]Chang, H.C., Huang, Y.C., Hung, W.C., 2003. Antiproliferative and chemopreventive effects of adlay seed on lung cancer in vitro and in vivo. J. Agric. Food Chem., 51(12):3656-3660.

[4]Chowdhury, S., 1946. Some studies on the smut Ustilago coicis Bref. of Job’s tears millet. J. Indian Bot. Soc., 25:123-130.

[5]Fullerton, R.A., Langdon, R.F.N., 1968. A study of some smuts of Echinochloa spp. Proc. Linnean Soc. New South Wales, 93:281-293.

[6]Kishino, H., Hasegawa, M., 1989. Evaluation of the maximum likelihood estimate of the evolutionary. J. Mol. Evol., 29(2):170-179.

[7]Mundkur, B.B., 1940. A second contribution towards a knowledge of Indian Ustilaginales: Fragments xxvi-l. Trans. Br. Mycol. Soc., 24(3-4):312-336.

[8]O′Donnell, K., 1993. Fusarium and its Near Relatives. In: Reynolds, D.R., Taylor, J.W. (Eds.), The Fungal Holomorph. Mitotic, Meiotic and Pleiomorphic Speciation in Fungal Systematics. CAB International, Wallingford, p.225-233.

[9]Page, R.D.M., 1996. TreeView: an application to display phylogenetic trees on personal computers. Comput. Appl. Biosci., 12(4):357-358.

[10]Piepenbring, M., 2003. Smut Fungi (Ustilaginomycetes p.p. and Microbotryales, Basidiomycota). Flora Neotropica Monograph 86. New York Botanical Garden Press, New York.

[11]Piepenbring, M., 2004. Comparative Morphology of Galls Formed by Smut Fungi and Discussion of Generic Concepts. In: Agerer, R., Piepenbring, M., Blanz, P. (Eds.), Frontiers in Basidiomycote Mycology. IHW, Eching, p.117-164.

[12]Piepenbring, M., Bauer, R., Oberwinkler, F., 1998. Teliospores of smut fungi: teliospore connections, appendages, and germ pores studied by electron microscopy; phylogenetic discussion of characteristics of teliospores. Protoplasma, 204(3-4):202-218.

[13]Small, W., 1927. Matter of phytopathological interest during 1926. Rev. Appl. Mycol., 6:273-274.

[14]Stoll, M., Piepenbring, M., Begerow, D., Oberwinkler, F., 2003. Molecular phylogeny of Ustilago and Sporisorium species (Basidiomycota, Ustilaginales) based on internal transcribed spacer (ITS) sequences. Can. J. Bot., 81(9):976-984.

[15]Stoll, M., Begerow, D., Oberwinkler, F., 2005. Molecular phylogeny of Ustilago, Sporisorium, and related taxa based on combined analyses of rDNA sequences. Mycol. Res., 109(3):342-356.

[16]Swofford, D.L., 2002. PAUP*: Phylogenetic Analysis Using Parsimony and Other Methods Version 4b10. Sinauer Associates, Sunderland, USA.

[17]Titatarn, S., Chiengkul, A., Unchalisangkas, D., Chamkrachang, W., Chew-Chin, N., Chandrasrikul, A., 1983. Occurrence of Ustilago coicis on Coix lachryma-jobi in Thailand. Plant Dis., 67(4):434-435.

[18]Vánky, K., 1985. Carpathian Ustilaginales. Symb. Bot. Upsal., 24:1-309.

[19]Vánky, K., 1987. Illustrated Genera of Smut Fungi. Gustav Fischer Verlag GmbH & Co. KG, Stuttgart, New York.

[20]Vánky, K., 1998. A survey of the spore-ball-forming smut fungi. Mycol. Res. 102(5):513-526.

[21]Xie, L., Zhang, J.Z., Wan, Y., Hu, D.W., 2010. Identification of Colletotrichum spp. isolated from strawberry in Zhejiang Province and Shanghai City, China. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 11(1):61-70.

[22]Zhang, J.Z., Chu, F.Q., Guo, D.P., Hyde, K.D., Xie, G.L., 2012. Cytology and ultrastructure of interactions between Ustilago esculenta and Zizania latifolia. Mycol. Progr., 11(2):499-508.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE