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CLC number: R781.05

On-line Access: 2013-05-04

Received: 2012-12-18

Revision Accepted: 2013-04-01

Crosschecked: 2013-04-15

Cited: 6

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.5 P.416-425

10.1631/jzus.B1200350


Short-term microbiological effects of scaling and root planing and essential-oils mouthwash in Chinese adults


Author(s):  Jia-yan He, Gang-gang Qi, Wu-jing Huang, Xu-dong Sun, Yu Tong, Chun-mei Peng, Xue-ping Zhou, Hui Chen

Affiliation(s):  Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   huic66@hotmail.com

Key Words:  Oral microbiota, Chronic periodontitis, Scaling and root planing, Essential-oils, Quantitative detection, Real-time PCR


Jia-yan He, Gang-gang Qi, Wu-jing Huang, Xu-dong Sun, Yu Tong, Chun-mei Peng, Xue-ping Zhou, Hui Chen. Short-term microbiological effects of scaling and root planing and essential-oils mouthwash in Chinese adults[J]. Journal of Zhejiang University Science B, 2013, 14(5): 416-425.

@article{title="Short-term microbiological effects of scaling and root planing and essential-oils mouthwash in Chinese adults",
author="Jia-yan He, Gang-gang Qi, Wu-jing Huang, Xu-dong Sun, Yu Tong, Chun-mei Peng, Xue-ping Zhou, Hui Chen",
journal="Journal of Zhejiang University Science B",
volume="14",
number="5",
pages="416-425",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200350"
}

%0 Journal Article
%T Short-term microbiological effects of scaling and root planing and essential-oils mouthwash in Chinese adults
%A Jia-yan He
%A Gang-gang Qi
%A Wu-jing Huang
%A Xu-dong Sun
%A Yu Tong
%A Chun-mei Peng
%A Xue-ping Zhou
%A Hui Chen
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 5
%P 416-425
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200350

TY - JOUR
T1 - Short-term microbiological effects of scaling and root planing and essential-oils mouthwash in Chinese adults
A1 - Jia-yan He
A1 - Gang-gang Qi
A1 - Wu-jing Huang
A1 - Xu-dong Sun
A1 - Yu Tong
A1 - Chun-mei Peng
A1 - Xue-ping Zhou
A1 - Hui Chen
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 5
SP - 416
EP - 425
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200350


Abstract: 
Objective: To assess the short-term effect of scaling and root planing (SRP) and essential-oils mouthwash on the levels of specific bacteria in Chinese adults. Methods: Fifty Chinese adults with chronic periodontitis were randomly assigned to full-mouth SRP or a 7-d essential-oils mouthwash regimen. In addition, 22 periodontally healthy adults used essential-oils mouthwash for 7 d. Clinical examination and plaque/saliva sampling were performed at baseline and on Day 7. Quantitative real-time polymerase chain reaction (PCR) was used to measure Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), and total bacterial loads in saliva, supra- and sub-gingival plaque samples. Results: The detection frequencies of four tested species remained unchanged after either treatment. However, the bacterial loads of Fn, Pg, and Pi were significantly reduced by SRP; the mean reduction of bacterial counts in saliva ranged from 52.2% to 62.5% (p<0.01), in supragingival plaque from 68.2% to 81.0% (p<0.05), and in subgingival plaque from 67.9% to 93.0% (p<0.01). Total bacterial loads were reduced after SRP in supra- and sub-gingival plaque (p<0.05). essential-oils mouthwash reduced Fn levels in supragingival plaque by a mean of 53.2%, and reduced total bacterial loads in supra- and sub-gingival plaque (p<0.01). In subgingival plaque from periodontal patients, Pg and Pi reductions were high after SRP compared to essential-oils mouthwash (93.0% vs. 37.7% and 87.0% vs. 21.0%, p<0.05). No significant bacterial reduction was observed in periodontally healthy subjects using essential-oils mouthwash. Conclusions: SRP and essential-oils mouthwash both have an impact on saliva and gingival plaque flora in Chinese periodontitis patients in 7 d, with greater microbiological improvement by SRP.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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