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On-line Access: 2013-06-03

Received: 2012-12-29

Revision Accepted: 2013-05-28

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.9 P.829-836

http://doi.org/10.1631/jzus.B1200363


Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences


Author(s):  Ya-e Zhao, Jun-xian Ma, Li Hu, Li-ping Wu, Manuel De Rojas

Affiliation(s):  Department of Immunology and Pathogen Biology, Xi’an Jiaotong University College of Medicine, Xi’an 710061, China; more

Corresponding email(s):   zhaoyae@mail.xjtu.edu.cn

Key Words:  Demodex folliculorum, cox1 partial sequences, Divergence, Genetic relationship, Phylogenetic tree


Ya-e Zhao, Jun-xian Ma, Li Hu, Li-ping Wu, Manuel De Rojas. Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences[J]. Journal of Zhejiang University Science B, 2013, 14(9): 829-836.

@article{title="Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences",
author="Ya-e Zhao, Jun-xian Ma, Li Hu, Li-ping Wu, Manuel De Rojas",
journal="Journal of Zhejiang University Science B",
volume="14",
number="9",
pages="829-836",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200363"
}

%0 Journal Article
%T Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences
%A Ya-e Zhao
%A Jun-xian Ma
%A Li Hu
%A Li-ping Wu
%A Manuel De Rojas
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 9
%P 829-836
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200363

TY - JOUR
T1 - Discrimination between Demodex folliculorum (Acari: Demodicidae) isolates from China and Spain based on mitochondrial cox1 sequences
A1 - Ya-e Zhao
A1 - Jun-xian Ma
A1 - Li Hu
A1 - Li-ping Wu
A1 - Manuel De Rojas
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 9
SP - 829
EP - 836
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200363


Abstract: 
For a long time, classification of Demodex mites has been based mainly on their hosts and phenotypic characteristics. A new subspecies of Demodex folliculorum has been proposed, but not confirmed. Here, cox1 partial sequences of nine isolates of three Demodex species from two geographical sources (China and Spain) were studied to conduct molecular identification of D. folliculorum. Sequencing showed that the mitochondrial cox1 fragments of five D. folliculorum isolates from the facial skin of Chinese individuals were 429 bp long and that their sequence identity was 97.4%. The average sequence divergence was 1.24% among the five Chinese isolates, 0.94% between the two geographical isolate groups (China (5) and Spain (1)), and 2.15% between the two facial tissue sources (facial skin (6) and eyelids (1)). The genetic distance and rate of third-position nucleotide transition/transversion were 0.0125, 2.7 (3/1) among the five Chinese isolates, 0.0094, 3.1 (3/1) between the two geographical isolate groups, and 0.0217, 4.4 (3/1) between the two facial tissue sources. phylogenetic trees showed that D. folliculorum from the two geographical isolate groups did not form sister clades, while those from different facial tissue sources did. According to the molecular characteristics, it appears that subspecies differentiation might not have occurred and that D. folliculorum isolates from the two geographical sources are of the same population. However, population differentiation might be occurring between isolates from facial skin and eyelids.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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