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Abstract: Objective: This study examined the anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidia and var. angustifolia, a natural slimming aid, on 3T3-L1 adipocytes. Methods: Methanol and water extracts of leaves of the F. deltoidea varieties were analyzed to determine their total flavonoid content (TFC) and total phenolic content (TPC), respectively. The study was initiated by determining the maximum non-toxic dose (MNTD) of the methanol and water extracts for 3T3-L1 preadipocytes. Possible anti-adipogenic effects were then examined by treating 2-d post confluent 3T3-L1 preadipocytes with either methanol extract or water extract at MNTD and half MNTD (½MNTD), after which the preadipocytces were induced to form mature adipocytes. Visualisation and quantification of lipid content in mature adipocytes were carried out through oil red O staining and measurement of optical density (OD) at 520 nm, respectively. Results: The TFCs of the methanol extracts were 1.36 and 1.97 g quercetin equivalents (QE)/100 g dry weight (DW), while the TPCs of the water extracts were 5.61 and 2.73 g gallic acid equivalents (GAE)/100 g DW for var. deltoidea and var. angustilofia, respectively. The MNTDs determined for methanol and water extracts were (300.0±28.3) and (225.0±21.2) μg/ml, respectively, for var. deltoidea, while much lower MNTDs [(60.0±2.0) μg/ml for methanol extracts and (8.0±1.0) μg/ml for water extracts] were recorded for var. angustifolia. Studies revealed that the methanol extracts of both varieties and the water extracts of var. angustifolia at either MNTD or ½MNTD significantly inhibited the maturation of preadipocytes. Conclusions: The inhibition of the formation of mature adipocytes indicated that leaf extracts of F. deltoidea could have potential anti-obesity effects.

金钱榕提取物抑制3T3-L1脂肪细胞抗脂肪沉积研究

研究目的：对两个品种金钱榕（var. deltoidea 和 var. angustifolia）叶片提取物抑制3T3-L1脂肪细胞脂肪沉积进行研究，为基于金钱榕的抗肥胖功能产品开发提供理论基础。
研究方法：用甲醇和水分别对两个品种金钱榕的叶片进行提取，并分别分析了提取物中的总黄酮含量（TFC）和总酚含量（TPC）。通过MTT法确定提取物对3T3-L1脂肪细胞的最大无毒剂量（MNTD）。然后，通过油红O染色和吸光度测定来定性和定量研究提取物对抗脂肪细胞脂肪沉积的作用。
重要结论：金钱榕两个品种deltoidea和angustifolia的甲醇提取物中的TFC含量分别为1.36和1.97 g QE/100 g DW，水提取物中的TPC含量分别为5.61和2.73 g GAE/100 g DW。金钱榕deltoidea品种的甲醇和水提取物对3T3-L1细胞的MNTD分别为（300.0±28.3）和（225.0±21.2）µg/ml，而angustifolia品种的MNTDs则较低（其甲醇和水提取物的MNTDs分别为（60.0±2.0）和（8.0±1.0）μg/ml）。金钱榕两个品种的甲醇提取物和angustifolia品种的水提取物在MNTD或½MNTD的浓度下，均具有显著抑制前脂肪细胞的成熟的作用。因此，根据其抑制成熟脂肪细胞形成的结果可以得出金钱榕叶片提取物具有潜在的抗肥胖效果。

关键词：脂肪细胞；金钱榕；黄酮类化合物；天然产物；肥胖

[1] Abdullah, M.A., Ahmed, K.A.A., Abuluhoom, F.M., 2010. Role of Ficus deltoidea extract in the enhancement of wound healing in experimental rats. Biomed Res, 21(3):241-245. 

[2] Abdullah, N.A.H., Karsani, S.A., Aminudin, N., 2008. Effects of Ficus deltoidea extract on the serum protein profile of simultaneously hypertensive rats. J Prot Bioinf, S2:143

[3] Abdullah, Z., Hussain, K., Zhari, I., 2011. Evaluation of extracts of leaf of three Ficus deltoidea varieties for antioxidant activities and secondary metabolites. Pharmacognosy Res, 1(4):216-223. 

[4] Adam, Z., Ismail, A., Khamis, S., 2011. Antihyperglycemic activity of F. deltoidea ethanolic extract in normal rats. Sains Malays, 40(5):489-495. 

[5] Ahn, J., Lee, H., Kim, S., 2008. The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways. Biochem Biophys Res Commun, 373(4):545-549. 

[6] Aris, S.R.S., Mustafa, S., Ahmat, N., 2009. Phenolic content and antioxidant activity of fruits of Ficus deltoidea var angustifolia sp. Malays. J Anal Sci, 13(2):146-150. 

[7] Chang, C.C., Yang, M.H., Wen, H.M., 2002. Estimation of total flavonoid content in propolis of two complementary colorimetric methods. J Food Drug Anal, 10(3):178-182. 

[8] Choi, I.W., Park, Y.K., Choi, H.D., 2006. Anti-adipogenic activity of rutin in 3T3-L1 cells and mice fed with high-fat diet. BioFactors, 26(4):273-281. 

[9] Filippini, R., Piovan, A., Borsarini, A., 2010. Study of dynamic accumulation of secondary metabolites in three subspecies of Hypericum perforatum . Fitoterapia, 81(2):115-119. 

[10] Gregoire, F.M., 2001. Adipocyte differentiation: from fibroblast to endocrine cell. Exp Biol Med, 226(11):997-1002. 

[11] Guigui, K., Beaudoin, A., 2007. The use of oil red O in sequence with other methods of fingerprint development. J Forensic Identif, 57(4):550-581. 

[12] Harmon, A.W., Harp, J.B., 2001. Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis. Am J Physiol Cell Physiol, 280(4):C807-C813. 

[13] Hsu, C.L., Yen, G.C., 2006. Induction of cell apoptosis in 3T3-L1 preadipocytes by flavonoids is associated with their antioxidant activity. Mol Nutr Food Res, 50(11):1072-1079. 

[14] Hsu, C.L., Yen, G.C., 2007. Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 adipocytes. J Agric Food Chem, 55(21):8404-8410. 

[15] Hsu, C.L., Huang, S.L., Yen, G.C., 2006. Inhibitory effect of phenolic acids on proliferation of 3T3-L1 preadipocytes in relation to their antioxidant activity. J Agric Food Chem, 54(12):4191-4197. 

[16] Ilyanie, Y., Wong, T.W., Choo, C.Y., 2010. Evaluation of hypoglycemic activity and toxicity profiles of the leaves of Ficus deltoidea in rodents. J Complem Integr Med, 8(1):Feb 2011

[17] Koo, H.M., Suhaila, M., 2001. Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. J Agric Food Chem, 49(6):3106-3112. 

[18] Laughton, M.J., Evans, P.J., Moroney, M.A., 1991. Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic dietary additives: relationship to antioxidant activity and to iron ion-reducing ability. Biochem Pharmacol, 42(9):1673-1681. 

[19] Lin, J.Y., Tang, C.Y., 2007. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem, 101(1):140-147. 

[20] Madsen, L., Petersen, R.K., Sorensen, M.B., 2003. Adipocyte differentiation of 3T3-L1 preadipocytes is dependent on lipoxygenase activity during the initial stages of the differentiation process. Biochem J, 375:539-549. 

[21] Moon, H.S., Chung, C.S., Lee, H.G., 2007. Inhibitory effect of (−)-epigallocatechin-3-gallate on lipid accumulation of 3T3-L1 cells. Obesity, 15(11):2571-2582. 

[22] Ong, S.L., Ling, A.P.K., Poospooragi, R., 2010. Production of flavonoid compounds in cell cultures of Ficus deltoidea as influenced by medium composition. Int J Med Aromatic Plants, 1(2):62-74. 

[23] Padwal, M.D., Majumdar, M.D., 2007. Drug treatments for obesity: orlistat, sibutramine, and rimonabant. Lancet, 369(9555):71-77. 

[24] Preuss, H.G., DiFerdinando, D., Bagchi, M., 2002.  Citrus aurantium as a thermogenic, weight-reduction replacement for ephedra: an overview. J Med, 33(1-4):247-264. 

[25] Rice-Evans, C.A., Miller, N.J., Paganga, G., 1996. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med, 20(7):933-956. 

[26] Sirisha, N., Sreenivasulu, M., Sangeeta, K., 2010. Antioxidant properties of Ficus species—a review. Int J PharmTech Res, 2(4):2174-2182. 

[27] Sukrasno, S., Irda, F., Kusnandar, A., 2011. Influence of drying method on flavonoid content of Cosmos caudatus (Kunth) leaves. Res J Med Plant, 5(2):189-195. 

[28] Sulaiman, M.R., Hussain, M.K., Zakaria, Z.A., 2008. Evaluation of the antinociceptive activity of Ficus deltoidea aqueous extract. Fitoterapia, 79(7-8):557-561. 

[29] Takenouchia, T., Takayamac, Y., Takezawaa, T., 2004. Co-treatment with dexamethasone and octanoate induces adipogenesis in 3T3-L1 cells. Cell Biol Int, 28(3):209-216. 

[30] Wolfram, S., Wang, Y., Thielecke, F., 2006. Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res, 50(2):176-187. 

[31] Yang, J.Y., Della-Fera, M.A., Diane, L.H., 2006. Esculetin induces apoptosis and inhibits adipogenesis in 3T3-L1 cells. Obesity, 14(10):1691-1699. 

[32] Yang, J.Y., Della-Fera, M.A., Rayalam, S., 2007. Effect of xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis. Apoptosis, 12(11):1953-1963. 

[33] Yasuko, S., Michael, F.C., Stephen, C.G., 2002. Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants. Toxicology, 177(1):67-80. 

[34] Zahra, S.F., Mahmood, A.A., Hapipah, M.A., 2009. Anti-ulcerogenic activity of aqueous extract of Ficus deltoidea against ethanol-induced gastric mucosal injury in rats. Res J Med Sci, 3(2):42-46.