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On-line Access: 2014-07-06

Received: 2013-12-18

Revision Accepted: 2014-05-11

Crosschecked: 2014-06-11

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.7 P.611-623

http://doi.org/10.1631/jzus.B1300320


Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.)*


Author(s):  Chang-ying Liu, Rui-hua L, Jun Li, Ai-chun Zhao, Xi-ling Wang, Umuhoza Diane, Xiao-hong Wang, Chuan-hong Wang, Ya-sheng Yu, Shu-mei Han, Cheng Lu, Mao-de Yu

Affiliation(s):  . State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China

Corresponding email(s):   yumd@163.com

Key Words:  Mulberry fruit, MaACS , MaACO , Gene expression, Abscisic acid, Ethephon


Chang-ying Liu, Rui-hua Lü, Jun Li, Ai-chun Zhao, Xi-ling Wang, Umuhoza Diane, Xiao-hong Wang, Chuan-hong Wang, Ya-sheng Yu, Shu-mei Han, Cheng Lu, Mao-de Yu. Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.)[J]. Journal of Zhejiang University Science B, 2014, 15(7): 611-623.

@article{title="Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.)",
author="Chang-ying Liu, Rui-hua Lü, Jun Li, Ai-chun Zhao, Xi-ling Wang, Umuhoza Diane, Xiao-hong Wang, Chuan-hong Wang, Ya-sheng Yu, Shu-mei Han, Cheng Lu, Mao-de Yu",
journal="Journal of Zhejiang University Science B",
volume="15",
number="7",
pages="611-623",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300320"
}

%0 Journal Article
%T Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.)
%A Chang-ying Liu
%A Rui-hua Lü
%A Jun Li
%A Ai-chun Zhao
%A Xi-ling Wang
%A Umuhoza Diane
%A Xiao-hong Wang
%A Chuan-hong Wang
%A Ya-sheng Yu
%A Shu-mei Han
%A Cheng Lu
%A Mao-de Yu
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 7
%P 611-623
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300320

TY - JOUR
T1 - Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.)
A1 - Chang-ying Liu
A1 - Rui-hua Lü
A1 - Jun Li
A1 - Ai-chun Zhao
A1 - Xi-ling Wang
A1 - Umuhoza Diane
A1 - Xiao-hong Wang
A1 - Chuan-hong Wang
A1 - Ya-sheng Yu
A1 - Shu-mei Han
A1 - Cheng Lu
A1 - Mao-de Yu
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 7
SP - 611
EP - 623
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300320


Abstract: 
1-Aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type I, MaACS3 and MaACS4 are type II, and MaACS5 is type III, with different C-terminal sequences. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demonstrated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid (ABA) and ethephon.

桑树MaACS和MaACO基因的鉴定和表达模式研究

研究目的:分离和鉴定桑树中参与乙烯生物合成的酶的编码基因MaACSMaACO,研究其表达模式。
创新要点:基于最新公布的桑树基因组数据库数据,获得5个MaACS基因和2个MaACO基因,对其进行了生物信息分析,同时鉴定了其在不同桑树组织中、不同发育时期桑椹中和不同激素作用下的表达模式。
研究方法:通过生物信息学方法筛选和鉴定基因,利用荧光定量逆转录聚合酶链式反应(qRT-PCR)分析基因的表达量。
重要结论:MaACSMaACO基因在根、茎、叶等不同组织中呈现出不同的表达模式,在桑椹发育过程中呈现出两种表达模式,其表达量被脱落酸和乙烯利上调。

关键词:桑椹;MaACS;MaACO;脱落酸;乙烯利

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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