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CLC number: X703

On-line Access: 2015-02-02

Received: 2014-03-27

Revision Accepted: 2014-11-06

Crosschecked: 2015-01-20

Cited: 3

Clicked: 2765

Citations:  Bibtex RefMan EndNote GB/T7714


An-cheng Luo


Xin Chen


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.2 P.145-154


Treatment of turtle aquaculture effluent by an improved multi-soil-layer system

Author(s):  Ying Song, Yu-ting Huang, Hong-fang Ji, Xin-jun Nie, Zhi-yuan Zhang, Chuan Ge, An-cheng Luo, Xin Chen

Affiliation(s):  MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   acluo@zju.edu.cn, cindy_chenx@163.com

Key Words:  Turtle aquaculture effluent, Multi-soil-layer (MSL) system, Sludge, Microbial community diversity

Ying Song, Yu-ting Huang, Hong-fang Ji, Xin-jun Nie, Zhi-yuan Zhang, Chuan Ge, An-cheng Luo, Xin Chen. Treatment of turtle aquaculture effluent by an improved multi-soil-layer system[J]. Journal of Zhejiang University Science B, 2015, 16(2): 145-154.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Treatment of turtle aquaculture effluent by an improved multi-soil-layer system
%A Ying Song
%A Yu-ting Huang
%A Hong-fang Ji
%A Xin-jun Nie
%A Zhi-yuan Zhang
%A Chuan Ge
%A An-cheng Luo
%A Xin Chen
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%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400090

T1 - Treatment of turtle aquaculture effluent by an improved multi-soil-layer system
A1 - Ying Song
A1 - Yu-ting Huang
A1 - Hong-fang Ji
A1 - Xin-jun Nie
A1 - Zhi-yuan Zhang
A1 - Chuan Ge
A1 - An-cheng Luo
A1 - Xin Chen
J0 - Journal of Zhejiang University Science B
VL - 16
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SP - 145
EP - 154
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1400090

Concentrated turtle aquaculture effluent poses an environmental threat to water bodies, and therefore needs to be treated prior to disposal. This study was conducted to assess the effect of multi-soil-layer (MSL) systems treating turtle aquaculture effluent with adding different amounts of sludge. Four MSL systems were constructed with dry weight ratios of sludge with 0%, 5%, 10%, and 20% (MSL 1, MSL 2, MSL 3, and MSL 4, respectively). The turtle aquaculture effluent had an average chemical oxygen demand (COD), ammonia nitrogen (NH4+-N) and total nitrogen (TN) concentration of 288.4, 213.4, and 252.0 mg/L, respectively. The COD/TN (C/N) ratio was 1.2. The results showed that the four MSL systems could effectively treat the COD, NH4+-N, and TN, and MSL 4 showed significantly improved NH4+-N removal efficiency, suggesting the potential of sludge addition to improve the turtle aquaculture effluent treatment. The average COD, TN, and NH4+-N removal efficiencies of MSL 4 were 70.3%, 66.5%, and 72.7%, respectively. To further interpret the contribution of microorganisms to the removal, the microbial community compositions and diversities of the four MSL systems were measured. Comparisons of the denaturing gradient gel electrophoresis (DGGE) profiles revealed that the amount of nitrifying bacteria and diversity in MSL 4 were higher than those in the other three systems. We concluded that adding 20% of sludge improved the NH4+-N removal and stability of the system for nitrification, due to the enrichment of the nitrifying bacteria in MSL 4.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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