Full Text:   <1549>

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CLC number: X703

On-line Access: 2015-02-02

Received: 2014-03-27

Revision Accepted: 2014-11-06

Crosschecked: 2015-01-20

Cited: 3

Clicked: 2765

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

An-cheng Luo

http://orcid.org/0000-0003-0404-1910

Xin Chen

http://orcid.org/0000-0003-1946-6006

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.2 P.145-154

http://doi.org/10.1631/jzus.B1400090


Treatment of turtle aquaculture effluent by an improved multi-soil-layer system


Author(s):  Ying Song, Yu-ting Huang, Hong-fang Ji, Xin-jun Nie, Zhi-yuan Zhang, Chuan Ge, An-cheng Luo, Xin Chen

Affiliation(s):  MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   acluo@zju.edu.cn, cindy_chenx@163.com

Key Words:  Turtle aquaculture effluent, Multi-soil-layer (MSL) system, Sludge, Microbial community diversity


Ying Song, Yu-ting Huang, Hong-fang Ji, Xin-jun Nie, Zhi-yuan Zhang, Chuan Ge, An-cheng Luo, Xin Chen. Treatment of turtle aquaculture effluent by an improved multi-soil-layer system[J]. Journal of Zhejiang University Science B, 2015, 16(2): 145-154.

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author="Ying Song, Yu-ting Huang, Hong-fang Ji, Xin-jun Nie, Zhi-yuan Zhang, Chuan Ge, An-cheng Luo, Xin Chen",
journal="Journal of Zhejiang University Science B",
volume="16",
number="2",
pages="145-154",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400090"
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%T Treatment of turtle aquaculture effluent by an improved multi-soil-layer system
%A Ying Song
%A Yu-ting Huang
%A Hong-fang Ji
%A Xin-jun Nie
%A Zhi-yuan Zhang
%A Chuan Ge
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%I Zhejiang University Press & Springer
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A1 - Yu-ting Huang
A1 - Hong-fang Ji
A1 - Xin-jun Nie
A1 - Zhi-yuan Zhang
A1 - Chuan Ge
A1 - An-cheng Luo
A1 - Xin Chen
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SP - 145
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1400090


Abstract: 
Concentrated turtle aquaculture effluent poses an environmental threat to water bodies, and therefore needs to be treated prior to disposal. This study was conducted to assess the effect of multi-soil-layer (MSL) systems treating turtle aquaculture effluent with adding different amounts of sludge. Four MSL systems were constructed with dry weight ratios of sludge with 0%, 5%, 10%, and 20% (MSL 1, MSL 2, MSL 3, and MSL 4, respectively). The turtle aquaculture effluent had an average chemical oxygen demand (COD), ammonia nitrogen (NH4+-N) and total nitrogen (TN) concentration of 288.4, 213.4, and 252.0 mg/L, respectively. The COD/TN (C/N) ratio was 1.2. The results showed that the four MSL systems could effectively treat the COD, NH4+-N, and TN, and MSL 4 showed significantly improved NH4+-N removal efficiency, suggesting the potential of sludge addition to improve the turtle aquaculture effluent treatment. The average COD, TN, and NH4+-N removal efficiencies of MSL 4 were 70.3%, 66.5%, and 72.7%, respectively. To further interpret the contribution of microorganisms to the removal, the microbial community compositions and diversities of the four MSL systems were measured. Comparisons of the denaturing gradient gel electrophoresis (DGGE) profiles revealed that the amount of nitrifying bacteria and diversity in MSL 4 were higher than those in the other three systems. We concluded that adding 20% of sludge improved the NH4+-N removal and stability of the system for nitrification, due to the enrichment of the nitrifying bacteria in MSL 4.

改良型多介质土壤渗滤系统对甲鱼养殖废水的净化效果研究

目的:通过向多介质土壤渗滤系统(MSL)的土壤层添加污泥,研究改进后的系统对工厂化甲鱼养殖废水的处理效果,分析系统内微生物群落的变化,以期部分揭示其可能的作用机理。
创新点:工厂化甲鱼养殖废水排放量日益增大,且水体氨氮含量较高。目前市场上缺乏针对甲鱼养殖废水的处理技术,MSL系统对该废水的处理也未有报道。本文在MSL系统的基础上进行改良,并将其应用于甲鱼养殖废水处理上,提出一套有效的甲鱼养殖废水处理技术,并对MSL系统内微生物群落结构进行了分析。
方法:向4套MSL小试装置中分别添加0%、5%、10%和20%污泥,研究其对工厂化甲鱼养殖废水的净化效果。试验中水质指标测定均按国家标准方法进行,系统内微生物群落结构采用聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)法测定。
结论:MSL系统可有效地处理工厂化甲鱼养殖废水,向系统中添加20%污泥后处理效果更佳。添加20%污泥的系统内具有较高的硝化类细菌多样性和都较多的生物量。

关键词:甲鱼养殖废水;多介质土壤渗滤系统;污泥;微生物群落结构

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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