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Abstract: Concentrated turtle aquaculture effluent poses an environmental threat to water bodies, and therefore needs to be treated prior to disposal. This study was conducted to assess the effect of multi-soil-layer (MSL) systems treating turtle aquaculture effluent with adding different amounts of sludge. Four MSL systems were constructed with dry weight ratios of sludge with 0%, 5%, 10%, and 20% (MSL 1, MSL 2, MSL 3, and MSL 4, respectively). The turtle aquaculture effluent had an average chemical oxygen demand (COD), ammonia nitrogen (NH₄⁺-N) and total nitrogen (TN) concentration of 288.4, 213.4, and 252.0 mg/L, respectively. The COD/TN (C/N) ratio was 1.2. The results showed that the four MSL systems could effectively treat the COD, NH₄⁺-N, and TN, and MSL 4 showed significantly improved NH₄⁺-N removal efficiency, suggesting the potential of sludge addition to improve the turtle aquaculture effluent treatment. The average COD, TN, and NH₄⁺-N removal efficiencies of MSL 4 were 70.3%, 66.5%, and 72.7%, respectively. To further interpret the contribution of microorganisms to the removal, the microbial community compositions and diversities of the four MSL systems were measured. Comparisons of the denaturing gradient gel electrophoresis (DGGE) profiles revealed that the amount of nitrifying bacteria and diversity in MSL 4 were higher than those in the other three systems. We concluded that adding 20% of sludge improved the NH₄⁺-N removal and stability of the system for nitrification, due to the enrichment of the nitrifying bacteria in MSL 4.

改良型多介质土壤渗滤系统对甲鱼养殖废水的净化效果研究

[1]Ahn, Y.H., 2006. Sustainable nitrogen elimination biotechnologies: a review. Process Biochem., 41(8):1709-1721.

[2]Boon, N., Windt, W., Verstraete, W., et al., 2002. Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. FEMS Microbiol. Ecol., 39(2):101-112.

[3]Chen, C.J., Huang, X.X., Lei, C.X., et al., 2012. Improving anammox start-up with bamboo charcoal. Chemosphere, 89(10):1224-1229.

[4]Chen, C.J., Huang, X.X., Lei, C.X., et al., 2013. Effect of organic matter strength on anammox for modified greenhouse turtle breeding wastewater treatment. Bioresour. Technol., 148:172-179.

[5]Chen, T.H., Lue, K.Y., 2010. Population status and distribution of freshwater turtles in Taiwan. Oryx, 44(2):261-266.

[6]Chen, X., Sato, K., Wakatsuki, T., et al., 2007. Effect of aeration and material composition in soil mixture block on the removal of colored substances and chemical oxygen demand in livestock wastewater using multi-soil-layering systems. Soil Sci. Plant Nutr., 53(4):509-516.

[7]Chen, X., Luo, A.C., Sato, K., et al., 2009. An introduction of a multi-soil-layering system: a novel green technology for wastewater treatment in rural areas. Water Environ. J., 23(4):255-262.

[8]Chu, L., Wang, J., 2011. Comparison of polyurethane foam and biodegradable polymer as carriers in moving bed biofilm reactor for treating wastewater with a low C/N ratio. Chemosphere, 83(1):63-68.

[9]da Silva, K.R., Wasielesky, W., Abreu, P.C., 2013. Nitrogen and phosphorus dynamics in the biofloc production of the pacific white shrimp, Litopenaeus vannamei. J. World Aquacult. Soc., 44(1):30-41.

[10]Environmental Protection Agency of China, 2002. Water and Wastewater Monitoring Method, 4th Ed. China Environmental Science Press, Beijing, China, p.211-279 (in Chinese).

[11]Feng, C., Sugiura, N., Shimada, S., et al., 2003. Development of a high performance electrochemical wastewater treatment system. J. Hazard. Mater., 103(1-2):65-78.

[12]Guan, Y.D., Chen, X., Zhang, S., et al., 2012. Performance of multi-soil-layering system (MSL) treating leachate from rural unsanitary landfills. Sci. Total Environ., 420:183-190.

[13]Iasur-Kruh, L., Hadar, Y., Milstein, D., et al., 2010. Microbial population and activity in wetland microcosms constructed for improving treated municipal wastewater. Microb. Ecol., 59(4):700-709.

[14]Isaacs, S.H., Henze, M., 1995. Controlled carbon source addition to an alternating nitrification-denitrification wastewater treatment process including biological P removal. Water Res., 29(1):77-89.

[15]Jung, J.Y., Chung, Y.C., Shin, H.S., et al., 2004. Enhanced ammonia nitrogen removal using consistent biological regeneration and ammonium exchange of zeolite in modified SBR process. Water Res., 38(2):347-354.

[16]Juretschko, S., Loy, A., Lehner, A., et al., 2002. The microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full-cycle rRNA approach. Syst. Appl. Microbiol., 25(1):84-99.

[17]Kim, D., Kim, T.S., Ryu, H.D., et al., 2008. Treatment of low carbon-to-nitrogen wastewater using two-stage sequencing batch reactor with independent nitrification. Process Biochem., 43(4):406-413.

[18]Kim, J.H., Chen, M., Kishida, N., et al., 2004. Integrated real-time control strategy for nitrogen removal in swine wastewater treatment using sequencing batch reactors. Water Res., 38(14-15):3340-3348.

[19]Kuba, T., van Loosdrecht, M., Heijnen, J., 1996. Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system. Water Res., 30(7):1702-1710.

[20]Lahav, O., Green, M., 1998. Ammonium removal using ion exchange and biological regeneration. Water Res., 32(7):2019-2028.

[21]Lao, S.G., 2001. Treatment of turtle-breeding wastewater and domestic fecal sewage with soil cultivating system. J. Environ. Sci. (China), 13(3):342-345.

[22]Li, H.Q., Han, H.J., Du, M.A., et al., 2011. Removal of phenols, thiocyanate and ammonium from coal gasification wastewater using moving bed biofilm reactor. Bioresour. Technol., 102(7):4667-4673.

[23]Lin, Y.F., Jing, S.R., Wang, T.W., et al., 2002. Effects of macrophytes and external carbon sources on nitrate removal from groundwater in constructed wetlands. Environ. Pollut., 119(3):413-420.

[24]Luanmanee, S., Attanandana, T., Masunaga, T., et al., 2001. The efficiency of a multi-soil-layering system on domestic wastewater treatment during the ninth and tenth years of operation. Ecol. Eng., 18(2):185-199.

[25]Meinhold, J., Pedersen, H., Arnold, E., et al., 1998. Effect of continuous addition of an organic substrate to the anoxic phase on biological phosphate removal. Water Sci. Tech., 38(1):97-105.

[26]Ministry of Agriculture and Fisheries Bureau of China, 2010. China Fisheries Statistical Yearbook. China Agriculture Press, Beijing, China, p.27 (in Chinese).

[27]Muyzer, G., de Waal, E.C., Uitterlinden, A.G., 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environl. Microbiol., 59(3):695-700.

[28]Ostace, G.S., Cristea, V.M., Agachi, P.S., 2011. Cost reduction of the wastewater treatment plant operation by MPC based on modified ASM1 with two-step nitrification/ denitrification model. Comput. Chem. Eng., 35(11):2469-2479.

[29]Pattnaik, R., Yost, R., Porter, G., et al., 2008. Improving multi-soil-layer (MSL) system remediation of dairy effluent. Ecol. Eng., 32(1):1-10.

[30]SEPA (State Environmental Protection Administration of China), 1987. GB/T 7479-1987: Water Quality— Determination of Ammonium-Nesster’s Reagent Colorimetric Method. National Standards of People’s Republic of China (in Chinese).

[31]SEPA (State Environmental Protection Administration of China), 1990a. GB/T 11894-1989: Water Quality— Determination of total Nitrogen—Alkaline Potassium Persulfate Digestion-UV Spectro Photometric Method. National Standards of People’s Republic of China (in Chinese).

[32]SEPA (State Environmental Protection Administration of China), 1990b. GB/T 11914-1989: Water Quality— Determination of the Chemical Oxygen Demand— Dichromate Method. National Standards of People’s Republic of China (in Chinese).

[33]SEPA (State Environmental Protection Administration of China), 2003. GB 18918-2002: Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant. National Standards of People’s Republic of China (in Chinese).

[34]Shi, H.T., Parham, J.F., Fan, Z.Y., et al., 2008. Short communication evidence for the massive scale of turtle farming in China. Oryx, 42(1):147-150.

[35]Siripong, S., Rittmann, B.E., 2007. Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants. Water Res., 41(5):1110-1120.

[36]Smith, V.H., Tilman, G.D., Nekola, J.C., 1999. Eutrophication: impacts of excess nutrient inputs on freshwater, marine, and terrestrial ecosystems. Environ. Pollut., 100(1-3):179-196.

[37]Sun, S.P., Pellicer-Nàcher, C., Merkey, B., et al., 2010. Effective biological nitrogen removal treatment processes for domestic wastewaters with low C/N ratios: a review. Environ. Eng. Sci., 27(2):111-126.

[38]Wakatsuki, T., Esumi, H., Omura, S., 1993. High performance and N & P-removable on-site domestic waste water treatment system by multi-soil-layering method. Water Sci. Technol., 27(1):31-40.

[39]Wen, Y., Chen, Y., Zheng, N., et al., 2010. Effects of plant biomass on nitrate removal and transformation of carbon sources in subsurface-flow constructed wetlands. Bioresour. Technol., 101(19):7286-7292.

[40]Wu, J., Zhang, J., Jia, W., et al., 2009. Impact of COD/N ratio on nitrous oxide emission from microcosm wetlands and their performance in removing nitrogen from wastewater. Bioresour. Technol., 100(12):2910-2917.

[41]Yuan, G.L., Liu, Y., 2001. Static purification of water-hyacinths on wastewater from intensive raising Chinese-turtle. Agro-Environ. Prot., 20(5):322-325 (in Chinese).

[42]Zhang, H.M., Xiao, J.N., Cheng, Y.J., et al., 2006. Comparison between a sequencing batch membrane bioreactor and a conventional membrane bioreactor. Process Biochem., 41(1):87-95.

[43]Zhao, L., Wang, Y., Yang, J., et al., 2010. Earthworm-microorganism interactions: a strategy to stabilize domestic wastewater sludge. Water Res., 44(8):2572-2582.