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CLC number: R735; R457

On-line Access: 2015-05-04

Received: 2014-08-11

Revision Accepted: 2015-01-22

Crosschecked: 2015-04-10

Cited: 2

Clicked: 4782

Citations:  Bibtex RefMan EndNote GB/T7714


Jin-ting Yang


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.5 P.395-403


Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes

Author(s):  Jin-ting Yang, Li-hui Tang, Yun-qing Liu, Yin Wang, Lie-ju Wang, Feng-jiang Zhang, Min Yan

Affiliation(s):  Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   zryanmin@zju.edu.cn

Key Words:  Erythrocytes, HepG2 cells, Intraoperative blood salvage, Cisplatin, Hyperthermia

Jin-ting Yang, Li-hui Tang, Yun-qing Liu, Yin Wang, Lie-ju Wang, Feng-jiang Zhang, Min Yan. Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes[J]. Journal of Zhejiang University Science B, 2015, 16(5): 395-403.

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author="Jin-ting Yang, Li-hui Tang, Yun-qing Liu, Yin Wang, Lie-ju Wang, Feng-jiang Zhang, Min Yan",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes
%A Jin-ting Yang
%A Li-hui Tang
%A Yun-qing Liu
%A Yin Wang
%A Lie-ju Wang
%A Feng-jiang Zhang
%A Min Yan
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 5
%P 395-403
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400224

T1 - Cisplatin combined with hyperthermia kills HepG2 cells in intraoperative blood salvage but preserves the function of erythrocytes
A1 - Jin-ting Yang
A1 - Li-hui Tang
A1 - Yun-qing Liu
A1 - Yin Wang
A1 - Lie-ju Wang
A1 - Feng-jiang Zhang
A1 - Min Yan
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 5
SP - 395
EP - 403
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400224

The safe use of intraoperative blood salvage (IBS) in cancer surgery remains controversial. Here, we investigated the killing effect of cisplatin combined with hyperthermia on human hepatocarcinoma (HepG2) cells and erythrocytes from IBS in vitro. hepG2 cells were mixed with concentrated erythrocytes and pretreated with cisplatin (50, 100, and 200 μg/ml) alone at 37 °C for 60 min and cisplatin (25, 50, 100, and 200 μg/ml) combined with hyperthermia at 42 °C for 60 min. After pretreatment, the cell viability, colony formation and DNA metabolism in HepG2 and the Na+-K+-ATPase activity, 2,3-diphosphoglycerate (2,3-DPG) concentration, free hemoglobin (Hb) level, osmotic fragility, membrane phosphatidylserine externalization, and blood gas variables in erythrocytes were determined. Pretreatment with cisplatin (50, 100, and 200 μg/ml) combined with hyperthermia (42 °C) for 60 min significantly decreased HepG2 cell viability, and completely inhibited colony formation and DNA metabolism when the HepG2 cell concentration was 5×104 ml−1 in the erythrocyte (P<0.01). Erythrocytic Na+-K+-ATPase activity, 2,3-DPG level, phosphatidylserine externalization, and extra-erythrocytic free Hb were significantly altered by hyperthermia plus high concentrations of cisplatin (100 and 200 μg/ml) (P<0.05), but not by hyperthermia plus 50 μg/ml cisplatin (P>0.05). In conclusion, pretreatment with cisplatin (50 μg/ml) combined with hyperthermia (42 °C) for 60 min effectively eliminated hepG2 cells from IBS but did not significantly affect erythrocytes in vitro.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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