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CLC number: R733.7

On-line Access: 2015-07-03

Received: 2014-08-26

Revision Accepted: 2015-01-28

Crosschecked: 2015-06-16

Cited: 4

Clicked: 2219

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Bo Wang

http://orcid.org/0000-0003-1158-7495

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.7 P.580-585

http://doi.org/10.1631/jzus.B1400234


Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro


Author(s):  Bo Wang, Sheng-yun Lin, Ying-ying Shen, Li-qiang Wu, Zhen-zhen Chen, Jing Li, Zhi Chen, Wen-bin Qian, Jian-ping Jiang

Affiliation(s):  Department of Hematology, the First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310006, China; more

Corresponding email(s):   w_bo1223@163.com, jiangjp77@163.com

Key Words:  Pure total flavonoid compounds, Human myeloid leukemia cells, Growth inhibition, Synergistic effect, Apoptosis


Bo Wang, Sheng-yun Lin, Ying-ying Shen, Li-qiang Wu, Zhen-zhen Chen, Jing Li, Zhi Chen, Wen-bin Qian, Jian-ping Jiang. Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro[J]. Journal of Zhejiang University Science B, 2015, 16(7): 580-585.

@article{title="Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro",
author="Bo Wang, Sheng-yun Lin, Ying-ying Shen, Li-qiang Wu, Zhen-zhen Chen, Jing Li, Zhi Chen, Wen-bin Qian, Jian-ping Jiang",
journal="Journal of Zhejiang University Science B",
volume="16",
number="7",
pages="580-585",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400234"
}

%0 Journal Article
%T Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro
%A Bo Wang
%A Sheng-yun Lin
%A Ying-ying Shen
%A Li-qiang Wu
%A Zhen-zhen Chen
%A Jing Li
%A Zhi Chen
%A Wen-bin Qian
%A Jian-ping Jiang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 7
%P 580-585
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400234

TY - JOUR
T1 - Pure total flavonoids from Citrus paradisi Macfadyen act synergistically with arsenic trioxide in inducing apoptosis of Kasumi-1 leukemia cells in vitro
A1 - Bo Wang
A1 - Sheng-yun Lin
A1 - Ying-ying Shen
A1 - Li-qiang Wu
A1 - Zhen-zhen Chen
A1 - Jing Li
A1 - Zhi Chen
A1 - Wen-bin Qian
A1 - Jian-ping Jiang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 7
SP - 580
EP - 585
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400234


Abstract: 
To investigate the potential effects of pure total flavonoid compounds (PTFCs) from Citrus paradisi Macfadyen separately or combined with arsenic trioxide on the proliferation of human myeloid leukemia cells and the mechanisms underlying the action of PTFCs. The effects of PTFCs separately or combined with arsenic trioxide on the proliferation and apoptosis of leukemia cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), fluorescence microscopy, and flow cytometry. Their effects on the expression levels of apoptosis-related regulators were determined by Western blot assay. PTFCs combined with arsenic trioxide significantly inhibited the growth of Kasumi-1 cells, and apoptosis was confirmed by flow cytometry analysis. Hoechst 33258 staining showed more significant morphological changes and more apoptosis following the combined treatment. Western blots showed changes in the expression of genes for poly ADP-ribose polymerase (PARP), caspase 3/9, and P65. The results indicated that PTFCs separately or combined with arsenic trioxide inhibited proliferation of leukemia cells in vitro and induced their apoptosis by modulating the expression of apoptosis-related regulator genes.

葡萄柚黄酮协同三氧化二砷诱导白血病细胞Kasumi-1凋亡的体外实验研究

目的:初步探索葡萄柚黄酮(PTFCs)联合三氧化二砷(As2O3)协同诱导白血病Kasumi-1细胞株凋亡及凋亡相关机制。
创新点:采用生物酶法与超声波辅助提取技术结合的方法从天然植物葡萄柚中提取和纯化出黄酮组分已取得国家发明型专利,发现其具有明显的抑制白血病细胞增殖作用,并且与As2O3存在协同作用。
方法:通过MTT法验证其抑制Kasumi-1细胞增殖作用,通过流式细胞术、Hoechst33258染色、蛋白质印迹(Westernblot)等技术探索其相关作用机制。
结论:发现PTFCs可以协同As2O3诱导Kasumi-1细胞凋亡。

关键词:葡萄柚黄酮;白血病细胞;抑制增殖;协同作用;凋亡

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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