Full Text:   <1064>

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CLC number: S821.5

On-line Access: 2015-06-08

Received: 2014-09-26

Revision Accepted: 2015-03-12

Crosschecked: 2015-04-16

Cited: 9

Clicked: 2029

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Tie-jun LI

http://orcid.org/0000-0002-6877-9693

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.496-502

http://doi.org/10.1631/jzus.B1400259


Effects of reducing dietary protein on the expression of nutrition sensing genes (amino acid transporters) in weaned piglets


Author(s):  Li Wu, Liu-qin He, Zhi-jie Cui, Gang Liu, Kang Yao, Fei Wu, Jun Li, Tie-jun Li

Affiliation(s):  Research Center of Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Corresponding email(s):   tjli@isa.ac.cn

Key Words:  Crude protein, Amino acid balance, Amino acid transporters


Li Wu, Liu-qin He, Zhi-jie Cui, Gang Liu, Kang Yao, Fei Wu, Jun Li, Tie-jun Li. Effects of reducing dietary protein on the expression of nutrition sensing genes (amino acid transporters) in weaned piglets[J]. Journal of Zhejiang University Science B, 2015, 16(6): 496-502.

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author="Li Wu, Liu-qin He, Zhi-jie Cui, Gang Liu, Kang Yao, Fei Wu, Jun Li, Tie-jun Li",
journal="Journal of Zhejiang University Science B",
volume="16",
number="6",
pages="496-502",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400259"
}

%0 Journal Article
%T Effects of reducing dietary protein on the expression of nutrition sensing genes (amino acid transporters) in weaned piglets
%A Li Wu
%A Liu-qin He
%A Zhi-jie Cui
%A Gang Liu
%A Kang Yao
%A Fei Wu
%A Jun Li
%A Tie-jun Li
%J Journal of Zhejiang University SCIENCE B
%V 16
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%P 496-502
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400259

TY - JOUR
T1 - Effects of reducing dietary protein on the expression of nutrition sensing genes (amino acid transporters) in weaned piglets
A1 - Li Wu
A1 - Liu-qin He
A1 - Zhi-jie Cui
A1 - Gang Liu
A1 - Kang Yao
A1 - Fei Wu
A1 - Jun Li
A1 - Tie-jun Li
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 496
EP - 502
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400259


Abstract: 
The effects of crude protein (CP) levels in the diet on the mRNA expression of amino acid (AA) transporters were studied in a 45-d trial. Eighteen piglets with an initial body weight (BW) of 9.57 kg were assigned to three groups (14%, 17%, and 20% CP in the diet) in a completely randomized design (six replicates per treatment). Diets were supplemented with crystalline AA to achieve equal standardized ileal digestible contents of Lys, Met plus Cys, Thr, and Trp, and were provided ad libitum. After 45 d, all piglets were slaughtered to collect small intestine samples. Compared with the values in the 14% CP group, the expressions of ASCT2, 4F2hc, and ATB0 mRNA in the jejunum were increased by 23.00%, 12.00%, 6.00% and 48.00%, 47.00%, 56.00% in the 17% and 20% CP groups, respectively. These results indicate that a 14% CP diet supplemented with crystalline AA may not transport enough AA into the body and maintain growth performance of piglets. However, a reduction of dietary 17% CP may reduce the excretion of nitrogen into the environment while supporting the development of piglets. Therefore, the 17% CP level is more suitable than 14% CP level.

降低日粮粗蛋白水平对断奶仔猪营养感受体基因(氨基酸转运载体)表达的影响

目的:验证日粮中粗蛋白质浓度对断奶仔猪氨基酸转运载体的影响效应。
创新点:从营养物质感应体的角度分析采用低氮日粮的可行性,并探索营养限制对营养转运体的影响及它们间的相互作用。
方法:十八头初始体重9.57公斤的断奶仔猪被随机分为三组(每组6个重复),分别饲喂含有14%、17%和20%的粗蛋白日粮45天。按照理想蛋白质模型,日粮分别添加赖氨酸、蛋氨酸+半胱氨酸、苏氨酸和色氨酸来满足断奶仔猪的需要,整个试验期自由采食。试验结束后,屠宰仔猪并采集小肠样品。与14%粗蛋白组相比,空肠中ASCT24F2hcATB0mRNA表达在17%和20%粗蛋白水平组分别上升23.00%、12.00%、6.00%和48.00%、47.00%、56.00%。
结论:结果表明,14%粗蛋白水平组外源添加合成氨基酸并不能促进氨基酸转运载体增加氨基酸的吸收来满足断奶仔猪的生长性能。然而,17%粗蛋白水平组可以减少氮排放到环境中,同时又能满足此阶段断奶仔猪的生长发育。因此,17%粗蛋白水平组对这个阶段断奶仔猪最合适。

关键词:粗蛋白;氨基酸平衡;氨基酸转运载体

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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