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CLC number: S811.2

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 2015-05-13

Cited: 3

Clicked: 4244

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Qing-biao Xu

http://orcid.org/0000-0001-8461-9924

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.503-510

http://doi.org/10.1631/jzus.B1400299


Duodenum has the greatest potential to absorb soluble non-ammonia nitrogen in the nonmesenteric gastrointestinal tissues of dairy cows


Author(s):  Ying-ming Xie, Qing-biao Xu, Yue-ming Wu, Xin-bei Huang, Jian-xin Liu

Affiliation(s):  Institute of Dairy Science, MoE Key Laboratory of Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   ymwu@zju.edu.cn

Key Words:  Soluble non-ammonia nitrogen, Nonmesenteric gastrointestine, Transporters, Dairy cows


Ying-ming Xie, Qing-biao Xu, Yue-ming Wu, Xin-bei Huang, Jian-xin Liu. Duodenum has the greatest potential to absorb soluble non-ammonia nitrogen in the nonmesenteric gastrointestinal tissues of dairy cows[J]. Journal of Zhejiang University Science B, 2015, 16(6): 503-510.

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author="Ying-ming Xie, Qing-biao Xu, Yue-ming Wu, Xin-bei Huang, Jian-xin Liu",
journal="Journal of Zhejiang University Science B",
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%T Duodenum has the greatest potential to absorb soluble non-ammonia nitrogen in the nonmesenteric gastrointestinal tissues of dairy cows
%A Ying-ming Xie
%A Qing-biao Xu
%A Yue-ming Wu
%A Xin-bei Huang
%A Jian-xin Liu
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T1 - Duodenum has the greatest potential to absorb soluble non-ammonia nitrogen in the nonmesenteric gastrointestinal tissues of dairy cows
A1 - Ying-ming Xie
A1 - Qing-biao Xu
A1 - Yue-ming Wu
A1 - Xin-bei Huang
A1 - Jian-xin Liu
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1400299


Abstract: 
In cattle, dietary protein is gradually degraded into peptide-bound amino acids (PBAAs), free amino acids (FAAs), and ultimately into ammonia by the rumen microbes. Both PBAA and FAA are milk protein precursors, and the rumen and small intestines are the main sites where such precursors are produced and absorbed. This work was designed to investigate the expression of the peptide transporter PepT1 and the AA transporters ASCT2, y+LAT1, and ATB0,+, and the concentrations of PBAA, FAA, and soluble protein in the rumen, omasum, and duodenum of dairy cows. Tissues and digesta were collected from six healthy Chinese Holstein dairy cows immediately after the animals were slaughtered. The expression of transporters was analyzed by real-time quantitative polymerase chain reaction (PCR). The FAA concentration was assessed using an amino acid (AA) analyzer, PBAA concentration by quantification of AA before and after acid-hydrolysis by 6 mol/L HCl, and soluble protein concentration by quantification of the bicinchoninic acid content. The results showed that the relative abundance of mRNA of the transporters and the soluble non-ammonia nitrogen (SNAN) concentration of each fraction were greater in the duodenum than in the rumen or omasum. These results indicate that the duodenum is the predominant location within the nonmesenteric digestive tract for producing milk protein precursors. In addition, PBAA was the largest component of SNAN in the digesta from the rumen, omasum, and duodenum. In conclusion, the duodenum has the greatest concentrations of SNAN and PBAA, and the greatest potential for absorption of SNAN in the form of PBAA in the nonmesenteric gastrointestinal tissues of dairy cows.

十二指肠在奶牛非肠系膜系统中吸收可溶性非氨态氮的潜在作用

目的:研究奶牛非肠系膜系统对可溶性非氨态氮的吸收能力。
创新点:目前国内外关于研究奶牛非肠系膜系统(瘤胃、瓣胃和十二指肠)内容物内可溶性非氨态氮和组织内转运载体表达的关系非常少。本文研究了瘤胃、瓣胃和十二指肠内可溶性非氨态氮的浓度,并检测了这些组织内小肽和氨基酸转运载体(PepT1ASCT2y+LAT1ATB0,+)的表达量,并进行了系统性的比较,在底物和基因之间建立了一定的联系。
方法:通过酸解法和氨基酸分析仪检测得到了瘤胃、瓣胃和十二指肠内可溶性非氨态氮的浓度(表3和4),并利用荧光定量聚合酶链反应(qRT-PCR)检测了各组织部位的载体基因表达量(图1)。
结论:十二指肠内容物的可溶性非氨态氮浓度最高,并且其载体的表达量也最大,表明十二指肠在奶牛非肠系膜系统中吸收可溶性非氨态氮的潜力最大,其中小肽在可溶性非氨态氮中占了大量比例。

关键词:可溶性非氨态氮;非肠系膜系统;转运载体;奶牛

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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