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CLC number: Q946

On-line Access: 2015-08-04

Received: 2014-11-11

Revision Accepted: 2015-04-30

Crosschecked: 2015-07-08

Cited: 6

Clicked: 2411

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Rowland Monday Ojo Kayode

http://orcid.org/0000-0002-2828-2519

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.8 P.680-689

http://doi.org/10.1631/jzus.B1400303


Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds


Author(s):  Rowland Monday Ojo Kayode, Anthony Jide Afolayan

Affiliation(s):  Medicinal Plant and Economic Development Research Centre, Department of Botany, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa

Corresponding email(s):   kayodermosnr@gmail.com, AAfolayan@ufh.ac.za

Key Words:  Moringa oleifera seed, Extraction methods, Essential oil, Cytotoxicity


Rowland Monday Ojo Kayode, Anthony Jide Afolayan. Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds[J]. Journal of Zhejiang University Science B, 2015, 16(8): 680-689.

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Abstract: 
Renewed interest in natural materials as food flavors and preservatives has led to the search for suitable essential oils. Moringa oleifera seed essential oil was extracted by solvent-free microwave and hydrodistillation. This study assessed its chemical constituents. cytotoxicity of the oils was investigated using hatchability and lethality tests on brine shrimps. A total of 16 and 26 compounds were isolated from the hydrodistillation extraction (HDE) and solvent-free microwave extraction (SME) oils, respectively, which accounted for 97.515% and 97.816% of total identifiable constituents, respectively. At 24 h when the most eggs had hatched, values of the SME (56.7%) and HDE (60.0%) oils were significantly different (P<0.05) from those of sea water (63.3%) and chloramphenicol (15.0%). Larva lethality was different significantly (P<0.05) between HDE and SME oils at different concentrations and incubation periods. The median lethal concentration (LC50) of the oils was >1000 mg/ml recommended as an index for non-toxicity, which gives the oil advantage over some antioxidant, antimicrobial, therapeutic, and preservative chemicals.

辣木籽精油的细胞毒性及其提取方法对化学成分的影响

目的:研究水蒸汽蒸馏法(HDE)和无溶剂微波萃取法(SME)对辣木籽精油化学组成的影响,并评估精油的细胞毒性。
创新点:比较了不同的提取精油方法对精油的化学成分及其细胞毒性的影响。
方法:分别采用HDE和SME两种方法提取辣木籽精油,然后使用气相色谱-质谱法分析其化学成分。通过比较在不同浓度和孵化时间下盐水虾卵的孵化率和幼虾的死亡率来检测精油的细胞毒性。
结论:HDE和SME两种方法提取的辣木籽精油分别含有16和26种化学成分,各自占总可识别成分的97.515%和97.816%。因此,SME提取法含有更多的成分。盐水虾卵分别在含有两种精油、海水和氯霉素的样品中孵化24 h后,孵化率分别为60.0%(HDE)、56.7%(SME)、63.3%(海水)和15.0%(氯霉素),精油中的孵化率远高于含有氯霉素的样品的孵化率(P<0.05)。同时,SME和HDE精油对幼虾的半致死量分别为2908.23和3473.63 µg/ml(大于1000 µg/ml被认为无毒)。因此,两种方法提取的精油可广泛适用于药物、食品防腐剂、抗氧化剂和香料等添加剂。

关键词:辣木籽;提取方法;精油;细胞毒性

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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