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CLC number: R394.6

On-line Access: 2015-07-03

Received: 2014-11-15

Revision Accepted: 2015-05-05

Crosschecked: 2015-06-16

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Xin-yue Liu


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.7 P.632-639


Correlation analysis of gene polymorphisms and β-lactam allergy

Author(s):  Jing Li, Xin-yue Liu, Lin-jing Li, Chong-ge You, Lei Shi, Shang-di Zhang, Qian Liu, Jun Wang, Ze-jing Liu, Ting-hong Lv

Affiliation(s):  Centre of Laboratory Medicine, Lanzhou University Second Hospital, Lanzhou 730030, China; more

Corresponding email(s):   liuxy@lzu.edu.cn

Key Words:  Allergy, β, -Lactam, Interleukin (IL), Pharmacogenomics, Single nucleotide polymorphism (SNP)

Jing Li, Xin-yue Liu, Lin-jing Li, Chong-ge You, Lei Shi, Shang-di Zhang, Qian Liu, Jun Wang, Ze-jing Liu, Ting-hong Lv. Correlation analysis of gene polymorphisms and β-lactam allergy[J]. Journal of Zhejiang University Science B, 2015, 16(7): 632-639.

@article{title="Correlation analysis of gene polymorphisms and β-lactam allergy",
author="Jing Li, Xin-yue Liu, Lin-jing Li, Chong-ge You, Lei Shi, Shang-di Zhang, Qian Liu, Jun Wang, Ze-jing Liu, Ting-hong Lv",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Correlation analysis of gene polymorphisms and β-lactam allergy
%A Jing Li
%A Xin-yue Liu
%A Lin-jing Li
%A Chong-ge You
%A Lei Shi
%A Shang-di Zhang
%A Qian Liu
%A Jun Wang
%A Ze-jing Liu
%A Ting-hong Lv
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 7
%P 632-639
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400309

T1 - Correlation analysis of gene polymorphisms and β-lactam allergy
A1 - Jing Li
A1 - Xin-yue Liu
A1 - Lin-jing Li
A1 - Chong-ge You
A1 - Lei Shi
A1 - Shang-di Zhang
A1 - Qian Liu
A1 - Jun Wang
A1 - Ze-jing Liu
A1 - Ting-hong Lv
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 7
SP - 632
EP - 639
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400309

A total of 64 patients with β;-Lactam allergy and 30 control subjects were enrolled in a case-control study. This study is aimed to analyze the relationship between β;-Lactam allergy and 10 single nucleotide polymorphisms (SNPs) in interleukin-10 (IL-10), IL-13, IL-4Rα, high-affinity immunoglobulin E-receptor β; chain (FcεRIβ;), interferon γ receptor 2 (IFNGR2), and CYP3A4, and within the Han Chinese population of Northwest China. Genotyping for the SNPs was conducted using the Sequenom MassARRAY® platform. SPSS 17.0 was employed to analyze the statistical data and SHEsis was used to perform the haplotype reconstruction and analyze linkage disequilibrium of SNPs of IL-10 and IL-13. The results showed that the genotype distribution of CYP3A4 rs2242480/CT differed significantly between case and control groups of males (P=0.022; odds ratio (OR)=0.167, 95% confidence interval (CI): 0.032–0.867). Further analysis showed that CCA, CCG, and TAA haplotypes of IL-10 had no significant correlation in patients with β;-Lactam allergy. The correlation between CCT and CAC haplotypes of IL-13 and β;-Lactam allergy needs to be further studied. The analysis did not reveal any differences in the distribution of others gene polymorphisms between cases and controls.


方法:以β-内酰胺类抗生素过敏者为研究对象进行病例对照研究,采用Sequenom MassARRAY®分子量阵列技术平台定制单核苷酸多态性(SNP)芯片检测10个单核苷酸多态性与甘肃地区汉族人群β-内酰胺类抗生素过敏易感性的关联性。应用SHEsis软件完成IL-10IL-13的连锁不平衡分析及单倍型构建。
结论:CYP3A4 rs2242480基因多态性与男性患者β-内酰胺类抗生素过敏有显著的相关性(P=0.022;OR=0.167,95% CI:0.032-0.867)。IL-13的CCT及CAC单倍型与中国西北地区汉族β-内酰胺类抗生素过敏的相关性有待进一步研究。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Apter, A.J., Schelleman, H., Walker, A., et al., 2008. Clinical and genetic risk factors of self-reported penicillin allergy. J. Allergy Clin. Immunol., 122(1):152-158.

[2]Bousquet, P.J., Demoly, P., Romano, A., 2009. Drug allergy and hypersensitivity: still a hot topic. Allergy, 64(2):179-182.

[3]Chen, Y., Zheng, T., Lan, Q., et al., 2011. Cytokine polymorphisms in Th1/Th2 pathway genes, body mass index, and risk of non-Hodgkin lymphoma. Blood, 117(2):585-590.

[4]Comte, D., Petitpierre, S., Spertini, F., et al., 2012. Allergy to β-lactam antibiotics. Rev. Med. Suisse, 8(337):836, 838-842 (in French).

[5]Ford, J.G., Rennick, D., Donaldson, D.D., et al., 2001. IL-13 and IFN-γ: interactions in lung inflammation. J. Immunol., 167(3):1769-1777.

[6]Gellner, K., Eiselt, R., Hustert, E., et al., 2001. Genomic organization of the human CYP3A locus: identification of a new, inducible CYP3A gene. Pharmacogenetics, 11(2):111-121.

[7]Gibson, G.G., Plant, N.J., Swales, K.E., et al., 2002. Receptor-dependent transcriptional activation of cytochrome P4503A genes: induction mechanisms, species differences and interindividual variation in man. Xenobiotica, 32(3):165-206.

[8]Guéant, J.L., Romano, A., Cornejo-Garcia, J.A., et al., 2015. HLA-DRA variants predict penicillin allergy in genome-wide fine-mapping genotyping. J. Allergy Clin. Immunol., 135(1):253-259.

[9]Guglielmi, L., Fontaine, C., Gougat, C., et al., 2006. IL-10 promoter and IL4-Rα gene SNPs are associated with immediate β-lactam allergy in atopic women. Allergy, 61(8):921-927.

[10]Hou, L., El-Omar, E.M., Chen, J., et al., 2007. Polymorphisms in Th1-type cell-mediated response genes and risk of gastric cancer. Carcinogenesis, 28(1):118-123.

[11]Jiang, X., 2015. Macrophage-produced IL-10 limits the chemotherapy efficacy in breast cancer. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 16(1):44-45.

[12]Kim, S.H., Park, H.S., 2006. Genetic markers for differentiating aspirin-hypersensitivity. Yonsei Med. J., 47(1):15-21.

[13]Kim, S.H., Choi, J.H., Park, H.S., 2005. Heterogeneity of the IgE response to allergenic determinants of cefaclor in serum samples from patients with cefaclor-induced anaphylaxis. Ann. Allergy Asthma Immunol., 94(6):700-704.

[14]Lee, Q.U., 2014. Use of cephalosporins in patients with immediate penicillin hypersensitivity: cross-reactivity revisited. Hong Kong Med. J., 20(5):428-436.

[15]Levine, S.J., Wenzel, S.E., 2010. Narrative review: the role of Th2 immune pathway modulation in the treatment of severe asthma and its phenotypes. Ann. Intern. Med., 152(4):232-237.

[16]Nagata, H., Mutoh, H., Kumahara, K., et al., 2001. Association between nasal allergy and a coding variant of the FcεRIβ gene Glu237Gly in a Japanese population. Hum. Genet., 109(3):262-266.

[17]Palomares, O., 2013. The role of regulatory T cells in IgE-mediated food allergy. J. Investig. Allergol. Clin. Immunol., 23(6):371-382.

[18]Prematta, T., Shah, S., Ishmael, F.T., 2012. Physician approaches to beta-lactam use in patients with penicillin hypersensitivity. Allergy Asthma Proc., 33(2):145-151.

[19]Purdue, M.P., Lan, Q., Kricker, A., et al., 2007. Polymorphisms in immune function genes and risk of non-Hodgkin lymphoma: findings from the New South Wales non-Hodgkin Lymphoma Study. Carcinogenesis, 28(3):704-712.

[20]Rubio, M., Bousquet, P.J., Demoly, P., 2010. IgE-mediated anaphylaxis to pristinamycin—report of a case. Allergy, 65(9):1198-1199.

[21]Sanak, M., Potaczek, D.P., Nizankowska-Mogilnicka, E., et al., 2007. Genetic variability of the high-affinity IgE receptor α subunit (FcεRIα) is related to total serum IgE levels in allergic subjects. Allergol. Int., 56(4):397-401.

[22]Shi, Y.Y., He, L., 2005. SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Res., 15(2):97-98.

[23]Torres, M.J., Mayorga, C., Leyva, L., et al., 2002. Controlled administration of penicillin to patients with a positive history but negative skin and specific serum IgE tests. Clin. Exp. Allergy, 32(2):270-276.

[24]Torres, M.J., Blanca, M., Fernandez, J., et al., 2003. Diagnosis of immediate allergic reactions to β-lactam antibiotics. Allergy, 58:961-972.

[25]Torres, M.J., Mayorga, C., Blanca-López, N., et al., 2014. Hypersensitivity reactions to β-lactams. EXS, 104: 165-184.

[26]Wu, L.C., Scheerens, H., 2014. Targeting IgE production in mice and humans. Curr. Opin. Immunol., 31:8-15.

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