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CLC number: S836

On-line Access: 2015-06-08

Received: 2014-11-30

Revision Accepted: 2015-03-18

Crosschecked: 2015-05-13

Cited: 10

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Citations:  Bibtex RefMan EndNote GB/T7714


Xiu-qi Wang


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.511-523


Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)

Author(s):  Ming-xia Chen, Xiang-guang Li, Jun-xian Yang, Chun-qi Gao, Bin Wang, Xiu-qi Wang, Hui-chao Yan

Affiliation(s):  College of Animal Science, South China Agricultural University / Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture / Guangdong Provincial Key Laboratory of Agro-Animal Genomics / South China Collaborative Innovatio Center for Poultry Disease Control and Product Safety, Guangzhou 510642, China; more

Corresponding email(s):   xqwang@scau.edu.cn, yanhc@scau.edu.cn

Key Words:  Embryonic growth, Nutrient transporters, Gene expression, Small intestine, Pigeons

Ming-xia Chen, Xiang-guang Li, Jun-xian Yang, Chun-qi Gao, Bin Wang, Xiu-qi Wang, Hui-chao Yan. Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)[J]. Journal of Zhejiang University Science B, 2015, 16(6): 511-523.

@article{title="Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)",
author="Ming-xia Chen, Xiang-guang Li, Jun-xian Yang, Chun-qi Gao, Bin Wang, Xiu-qi Wang, Hui-chao Yan",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)
%A Ming-xia Chen
%A Xiang-guang Li
%A Jun-xian Yang
%A Chun-qi Gao
%A Bin Wang
%A Xiu-qi Wang
%A Hui-chao Yan
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 511-523
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400340

T1 - Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)
A1 - Ming-xia Chen
A1 - Xiang-guang Li
A1 - Jun-xian Yang
A1 - Chun-qi Gao
A1 - Bin Wang
A1 - Xiu-qi Wang
A1 - Hui-chao Yan
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 511
EP - 523
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400340

The objective of this study was to investigate the relationship between gene expression of nutrient (amino acid, peptide, sodium and proton) transporters in the small intestine and embryonic growth in domestic pigeons (Columba livia). One hundred and twenty-five fertilized eggs were randomly assigned into five groups and were incubated under optimal conditions (temperature of 38.1 °C and relative humidity of 55%). Twenty embryos/birds from each group were sacrificed by cervical dislocation on embryonic day (E) 9, 11, 13, 15 and day of hatch (DOH). The eggs, embryos (without yolk sac), and organs (head, brain, heart, liver, lungs, kidney, gizzard, small intestine, legs, and thorax) were dissected, cleaned, and weighed. small intestine samples were collected for RNA isolation. The mRNA abundance of intestinal nutrient transporters was evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR). We classified these ten organs into four types according to the changes in relative weight during embryonic development. In addition, the gene expression of nutrient transporters was differentially regulated by embryonic day. The mRNA abundances of b0,+AT, EAAT3, y+LAT2, PepT1, LAT4, NHE2, and NHE3 increased linearly with age, whereas mRNA abundances of CAT1, CAT2, LAT1, EAAT2, SNAT1, and SNAT2 were increased to higher levels on E9 or E11 and then decreased to lower levels until DOH. The results of correlation analysis showed that the gene expressions of b0,+AT, EAAT3, PepT1, LAT4, NHE2, NHE3, and y+LAT2 had positive correlations with body weight (0.71<correlation coefficient (CC)<0.82, P<0.0001), while CAT1, CAT2, EAAT2, SNAT1, and SNAT2 had negative correlations with body weight (−0.86<CC<−0.64, P<0.0001). The gene expressions of b0,+AT, EAAT3, LAT4, PepT1, NHE2, NHE3, and y+LAT2 showed positive correlations with intestinal weight (0.80<CC<0.91, P<0.0001), while CAT1, CAT2, and EAAT2 showed negative correlations with intestinal weight (−0.84<CC<−0.67, P<0.0001). It was concluded that the differences between growth trajectories of organs and gene expression of nutrient transporters in small intestine were due to their functional and physiological properties, which provided a comprehensive study of amino acid and peptide transporter mRNA in the small intestine during embryonic growth of pigeons.




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