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CLC number: Q182
On-line Access: 2015-10-03
Received: 2015-03-09
Revision Accepted: 2015-06-09
Crosschecked: 2015-09-13
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Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene
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Qi-chao Zhao, Ming-hong Liu, Xian-wen Zhang, Chao-yang Lin, Qing Zhang, Zhi-cheng Shen. Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene[J]. Journal of Zhejiang University Science B, 2015, 16(10): 824-831.
@article{title="Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene",
author="Qi-chao Zhao, Ming-hong Liu, Xian-wen Zhang, Chao-yang Lin, Qing Zhang, Zhi-cheng Shen",
journal="Journal of Zhejiang University Science B",
volume="16",
number="10",
pages="824-831",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500056"
}
%0 Journal Article
%T Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene
%A Qi-chao Zhao
%A Ming-hong Liu
%A Xian-wen Zhang
%A Chao-yang Lin
%A Qing Zhang
%A Zhi-cheng Shen
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 10
%P 824-831
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500056
TY - JOUR
T1 - Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene
A1 - Qi-chao Zhao
A1 - Ming-hong Liu
A1 - Xian-wen Zhang
A1 - Chao-yang Lin
A1 - Qing Zhang
A1 - Zhi-cheng Shen
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 10
SP - 824
EP - 831
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500056
Abstract: Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.
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