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CLC number: Q815

On-line Access: 2015-12-04

Received: 2015-06-17

Revision Accepted: 2015-08-04

Crosschecked: 2015-11-11

Cited: 4

Clicked: 1982

Citations:  Bibtex RefMan EndNote GB/T7714


Guo-qing He


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.12 P.998-1010


Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS

Author(s):  Mei-lin Cui, Huan-yi Yang, Guo-qing He

Affiliation(s):  Zhejiang Provincial Key Laboratory of Food Microbiology, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   gqhe@zju.edu.cn

Key Words:  Ganoderma lucidum, Wort, Response surface methodology, Ganoderic acids, HPLC-ESI-MS

Mei-lin Cui, Huan-yi Yang, Guo-qing He. Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS[J]. Journal of Zhejiang University Science B, 2015, 16(12): 998-1010.

@article{title="Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS",
author="Mei-lin Cui, Huan-yi Yang, Guo-qing He",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS
%A Mei-lin Cui
%A Huan-yi Yang
%A Guo-qing He
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 12
%P 998-1010
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500147

T1 - Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS
A1 - Mei-lin Cui
A1 - Huan-yi Yang
A1 - Guo-qing He
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 12
SP - 998
EP - 1010
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500147

As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H2O and CO2, as well as cleavage of the D-ring.


结论:本实验响应面结果显示:当培养基含麦芽汁4.1%,酵母浸出粉1.89%,pH为5.40时,灵芝液体发酵菌丝生物量及胞内三萜产量均达到最优,分别为1.87 g/100 ml和93.21 mg/100 ml。经HPLC-ESI-MS鉴定,初步确定其含有10种灵芝酸成分。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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