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CLC number: Q815

On-line Access: 2015-12-04

Received: 2015-06-17

Revision Accepted: 2015-08-04

Crosschecked: 2015-11-11

Cited: 4

Clicked: 1982

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Guo-qing He

http://orcid.org/0000-0002-1177-8016

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.12 P.998-1010

http://doi.org/10.1631/jzus.B1500147


Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS


Author(s):  Mei-lin Cui, Huan-yi Yang, Guo-qing He

Affiliation(s):  Zhejiang Provincial Key Laboratory of Food Microbiology, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   gqhe@zju.edu.cn

Key Words:  Ganoderma lucidum, Wort, Response surface methodology, Ganoderic acids, HPLC-ESI-MS


Mei-lin Cui, Huan-yi Yang, Guo-qing He. Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS[J]. Journal of Zhejiang University Science B, 2015, 16(12): 998-1010.

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author="Mei-lin Cui, Huan-yi Yang, Guo-qing He",
journal="Journal of Zhejiang University Science B",
volume="16",
number="12",
pages="998-1010",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500147"
}

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%T Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS
%A Mei-lin Cui
%A Huan-yi Yang
%A Guo-qing He
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500147

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T1 - Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS
A1 - Mei-lin Cui
A1 - Huan-yi Yang
A1 - Guo-qing He
J0 - Journal of Zhejiang University Science B
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SP - 998
EP - 1010
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500147


Abstract: 
As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% (0.041 g/ml) and yeast extract 1.89% (0.0189 g/ml), pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H2O and CO2, as well as cleavage of the D-ring.

利用液体发酵提高灵芝三萜产量及HPLC-ESI-MS鉴定灵芝菌丝中灵芝酸成分

目的:通过优化液体发酵培养基提高灵芝三萜产量,并对其三萜成分进行初步鉴定。
创新点:麦芽作为啤酒生产原料,含有丰富的糖类及其他营养物质,并且价格低廉,来源广泛。本文首次将麦芽汁作为碳源物质以提高灵芝三萜产量,并对其所含灵芝酸成分进行初步鉴定。
方法:以灵芝菌丝生物量和胞内三萜产量为检测指标,对其液体发酵培养基中的碳(C)源和氮(N)源进行筛选,并利用单因素实验确定其相应浓度,再经响应面法优化液体发酵培养基(C源、N源、pH)(表1)。另一方面,酸碱转化法纯化灵芝三萜提取物后,利用高效液相色谱-电喷雾-质谱(HPLC-ESI-MS)法初步鉴定灵芝所含灵芝酸成分(表2)。
结论:本实验响应面结果显示:当培养基含麦芽汁4.1%,酵母浸出粉1.89%,pH为5.40时,灵芝液体发酵菌丝生物量及胞内三萜产量均达到最优,分别为1.87 g/100 ml和93.21 mg/100 ml。经HPLC-ESI-MS鉴定,初步确定其含有10种灵芝酸成分。

关键词:灵芝;麦芽汁;响应面;灵芝酸;高效液相色谱-电喷雾-质谱(HPLC-ESI-MS)

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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