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CLC number: Q946.2

On-line Access: 2016-02-01

Received: 2015-08-29

Revision Accepted: 2015-11-30

Crosschecked: 2016-01-13

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Qing-Yao Shu


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.2 P.100-109


Tissue-specific expression, developmentally and spatially regulated alternative splicing, and protein subcellular localization of OsLpa rice

Author(s):  Hai-ping Lu, Wei-qin Pang, Wen-xu Li, Yuan-yuan Tan, Qing Wang, Hai-Jun Zhao, Qing-Yao Shu

Affiliation(s):  State Key Laboratory of Rice Biology, Institution of Crop Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   qyshu@zju.edu.cn

Key Words:  OsLpa1, Low phytic acid, Expression pattern, Alternative splicing, Subcellular localization

Hai-ping Lu, Wei-qin Pang, Wen-xu Li, Yuan-yuan Tan, Qing Wang, Hai-Jun Zhao, Qing-Yao Shu. Tissue-specific expression, developmentally and spatially regulated alternative splicing, and protein subcellular localization of OsLpa rice[J]. Journal of Zhejiang University Science B, 2016, 17(2): 100-109.

@article{title="Tissue-specific expression, developmentally and spatially regulated alternative splicing, and protein subcellular localization of OsLpa rice",
author="Hai-ping Lu, Wei-qin Pang, Wen-xu Li, Yuan-yuan Tan, Qing Wang, Hai-Jun Zhao, Qing-Yao Shu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Tissue-specific expression, developmentally and spatially regulated alternative splicing, and protein subcellular localization of OsLpa rice
%A Hai-ping Lu
%A Wei-qin Pang
%A Wen-xu Li
%A Yuan-yuan Tan
%A Qing Wang
%A Hai-Jun Zhao
%A Qing-Yao Shu
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 2
%P 100-109
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500205

T1 - Tissue-specific expression, developmentally and spatially regulated alternative splicing, and protein subcellular localization of OsLpa rice
A1 - Hai-ping Lu
A1 - Wei-qin Pang
A1 - Wen-xu Li
A1 - Yuan-yuan Tan
A1 - Qing Wang
A1 - Hai-Jun Zhao
A1 - Qing-Yao Shu
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 2
SP - 100
EP - 109
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500205

The OsLpa1 gene (LOC_Os57400) was identified to be involved in phytic acid (PA) metabolism because its knockout and missense mutants reduce PA content in rice grain. However, little is known about the molecular characteristics of OsLpa rice and of its homologues in other plants. In the present study, the spatial pattern of OsLpa1 expression was revealed using OsLpa1 promoter::GUS transgenic plants (GUS: β-glucuronidase); GUS histochemical assay showed that OsLpa1 was strongly expressed in stem, leaf, and root tissues, but in floral organ it is expressed mainly and strongly in filaments. In seeds, GUS staining was concentrated in the aleurone layers; a few blue spots were observed in the outer layers of embryo, but no staining was observed in the endosperm. Three OsLpa1 transcripts (OsLpa1.1, OsLpa1.2, OsLpa1.3) are produced due to alternative splicing; quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) analysis revealed that the abundance of OsLpa1.3 was negligible compared with OsLpa1.1 and OsLpa all tissues. OsLpa1.2 is predominant in germinating seeds (about 5 times that of OsLpa1.1), but its abundance decreases quickly with the development of seedlings and plants, whereas the abundance of OsLpa1.1 rises and falls, reaching its highest level in 45-d-old plants, with abundance greater than that of OsLpa both leaves and roots. In seeds, the abundance of OsLpa1 continuously increases with seed growth, being 27.5 and 15 times greater in 28-DAF (day after flowering) seeds than in 7-DAF seeds for OsLpa1.1 and OsLpa1.2, respectively. Transient expression of chimeric genes with green fluorescence protein (GFP) in rice protoplasts demonstrated that all proteins encoded by the three OsLpa1 transcripts are localized to the chloroplast.




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