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CLC number: S852

On-line Access: 2017-04-05

Received: 2015-09-14

Revision Accepted: 2016-06-23

Crosschecked: 2017-03-13

Cited: 0

Clicked: 1731

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Song-hua Hu

http://orcid.org/0000-0002-8266-6791

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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.4 P.360-364

10.1631/jzus.B1500222


Efficacy of vaccination and nisin Z treatments to eliminate intramammary Staphylococcus aureus infection in lactating cows


Author(s):  Ran Guan, Jun-qiang Wu, Wei Xu, Xiao-yan Su, Song-hua Hu

Affiliation(s):  Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   songhua@zju.edu.cn

Key Words:  Subclinical mastitis, Intramammary infection, Staphylococcus aureus, Bacterin, Nisin


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Ran Guan, Jun-qiang Wu, Wei Xu, Xiao-yan Su, Song-hua Hu. Efficacy of vaccination and nisin Z treatments to eliminate intramammary Staphylococcus aureus infection in lactating cows[J]. Journal of Zhejiang University Science B, 2017, 18(4): 360-364.

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author="Ran Guan, Jun-qiang Wu, Wei Xu, Xiao-yan Su, Song-hua Hu",
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doi="10.1631/jzus.B1500222"
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%A Ran Guan
%A Jun-qiang Wu
%A Wei Xu
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%A Song-hua Hu
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500222

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T1 - Efficacy of vaccination and nisin Z treatments to eliminate intramammary Staphylococcus aureus infection in lactating cows
A1 - Ran Guan
A1 - Jun-qiang Wu
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J0 - Journal of Zhejiang University Science B
VL - 18
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1500222


Abstract: 
This study evaluated the effect of a Staphylococcus aureus bacterin and nisin on bovine subclinical mastitis. A total of 75 Holstein subclinically mastitic cows were randomly allocated to three groups with 25 cows per group. In group I, an intramammary infusion of nisin Z at a dose of 2.50×106 IU was carried out once daily for three days, and an autogenous S. aureus bacterin was inoculated into the supramammary lymph node one week before and one week after nisin treatment. In group II, nisin was administered in the same way as in group I, but no bacterin was inoculated. Group III received no treatment and served as a control. Milk was aseptically sampled from the affected quarters before and 2, 4, and 6 weeks after treatment, for bacteriological examination and analyses of N-acetyl-β-D-glucosaminidase (NAGase) activity, somatic cell count (SCC), and milk protein and fat contents. Results indicated that, compared to the nisin-treated group, nisin-bacterin treatment significantly reduced intramammary S. aureus infections, reduced the number of quarters with milk SCCs of more than 5×105 cells/ml, and increased the protein and fat contents of the milk. Therefore, nisin-bacterin therapy is suggested when subclinical mastitis occurs in lactating cows.

疫苗接种与乳酸链球菌素Z治疗对泌乳期奶牛乳腺感染金黄色葡萄球菌的疗效研究

目的:评价联合应用金黄色葡萄球菌疫苗接种和乳酸链球菌素Z治疗对乳腺隐性感染金黄色葡萄球菌奶牛的治疗效果。
创新点:首次证明了金黄色葡萄球菌疫苗接种和乳酸链球菌Z治疗对泌乳期奶牛乳腺金黄色葡萄球菌感染的治疗效果。
方法:将75头乳腺隐性感染金黄色葡萄球菌的泌乳期黑白花奶牛随机分为3组:金黄色葡萄球菌疫苗接种+乳酸链球菌素治疗组、乳酸链球菌素治疗组和不治疗对照组。在治疗前和治疗后2、4和 6周,对无菌采集感染乳区奶样进行细菌学检查、牛奶体细胞计数、N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)活性检测和牛奶品质(乳脂率和蛋白率)检测。
结论:和乳酸链球菌素单独治疗比较,金黄色葡萄球菌疫苗接种联合乳酸链球菌素治疗显著降低了感染乳腺和高体细胞数乳腺的数量。因此,金黄色葡萄球菌疫苗接种联合乳酸链球菌素治疗对乳腺感染金黄色葡萄球菌奶牛的治疗效果优于乳酸链球菌素单独治疗。

关键词:隐性乳房炎;乳腺感染;金黄色葡萄球菌;菌苗;乳酸链球菌素

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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