CLC number: Q2-33
On-line Access: 2016-10-02
Received: 2015-09-25
Revision Accepted: 2016-02-16
Crosschecked: 2016-09-10
Cited: 1
Clicked: 3654
Yuan-yuan Xu, Yang-yang Sun, Yu-juan Zhang, Chen-he Lu, Jin-feng Miao. Detection of biological thiols based on a colorimetric method[J]. Journal of Zhejiang University Science B, 2016, 17(10): 807-812.
@article{title="Detection of biological thiols based on a colorimetric method",
author="Yuan-yuan Xu, Yang-yang Sun, Yu-juan Zhang, Chen-he Lu, Jin-feng Miao",
journal="Journal of Zhejiang University Science B",
volume="17",
number="10",
pages="807-812",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500232"
}
%0 Journal Article
%T Detection of biological thiols based on a colorimetric method
%A Yuan-yuan Xu
%A Yang-yang Sun
%A Yu-juan Zhang
%A Chen-he Lu
%A Jin-feng Miao
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 10
%P 807-812
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500232
TY - JOUR
T1 - Detection of biological thiols based on a colorimetric method
A1 - Yuan-yuan Xu
A1 - Yang-yang Sun
A1 - Yu-juan Zhang
A1 - Chen-he Lu
A1 - Jin-feng Miao
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 10
SP - 807
EP - 812
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500232
Abstract: Biological thiols (biothiols), an important kind of functional biomolecules, such as cysteine (Cys) and glutathione (GSH), play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.
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