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CLC number: X173; X53

On-line Access: 2016-06-03

Received: 2015-10-08

Revision Accepted: 2015-12-18

Crosschecked: 2016-05-12

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hong-yun Peng

http://orcid.org/0000-0001-5520-7074

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.6 P.447-454

http://doi.org/10.1631/jzus.B1500242


Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens


Author(s):  Hong-yun Peng, Xue-hong Zhang, Jin-zhong Xu

Affiliation(s):  Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   penghongyun@zju.edu.cn

Key Words:  Chromatographic separation, Copper-tolerant plant, Elsholtzia splendens, Flavonoid glycoside, Structure elucidation


Hong-yun Peng, Xue-hong Zhang, Jin-zhong Xu. Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens[J]. Journal of Zhejiang University Science B, 2016, 17(6): 447-454.

@article{title="Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens",
author="Hong-yun Peng, Xue-hong Zhang, Jin-zhong Xu",
journal="Journal of Zhejiang University Science B",
volume="17",
number="6",
pages="447-454",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500242"
}

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%T Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens
%A Hong-yun Peng
%A Xue-hong Zhang
%A Jin-zhong Xu
%J Journal of Zhejiang University SCIENCE B
%V 17
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%P 447-454
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500242

TY - JOUR
T1 - Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens
A1 - Hong-yun Peng
A1 - Xue-hong Zhang
A1 - Jin-zhong Xu
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 6
SP - 447
EP - 454
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500242


Abstract: 
Elsholtzia splendens (Lamiaceae) is a copper-tolerant plant species growing on copper deposits in the south of China. chromatographic separation of n-BuOH extracts from the flowering aerial biomass afforded apigenin-7-O-β-D-glycoside, using macroporous resin, Sephadex™ LH-20 gel, polyamide resin as well as preparative high-performance liquid chromatography (P-HPLC) columns. Chemical structure was elucidated using HPLC/ESI-MS (electrospray ionization-mass spectrometry), Fourier transform infrared (FTIR), and 1D- and 2D-nuclear magnetic resonance (NMR). Apigenin-7-O-β-D-glycoside could be the post-harvesting product from E. splendens biomass.

从铜耐性植物海州香薷中提取分离芹菜素糖苷

目的:建立从铜耐性植物海州香薷地上部提取分离芹菜素糖苷的工艺技术,探索铜耐性植物海州香薷资源化途径。
创新点:结合使用大孔树脂、凝胶树脂、聚酰胺树脂多种色谱柱技术,从铜耐性植物海州香薷中提取分离纯度达98%的黄酮苷类化合物芹菜素糖苷。
方法:以铜耐性植物海州香薷为原料,用60%乙醇溶液浸提,提取液依次使用石油醚、乙酸乙酯、饱和正丁醇萃取。实验中,对饱和正丁醇萃取层,使用D101型大孔吸附树脂粗分离提取,Sephadex™ LH-20型凝胶筛分分离,并借助半制备液相和聚酰胺树脂分离纯化,得到芹菜素糖苷单体(图1)。利用紫外光谱、液相色谱–质谱联用、红外光谱、一维和二维核磁图谱,鉴定芹菜素糖苷结构(图2~5)。
结论:芹菜素糖苷是铜耐性植物海州香薷体内的天然产物,利用色谱技术可以从海州香薷植物体中分离出芹菜素糖苷单体。

关键词:色谱分离;铜耐性植物;海州香薷;黄酮苷;结构解析

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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