CLC number: S85
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2016-05-12
Cited: 2
Clicked: 4717
Chen Mei, Sha-sha He, Peng Yin, Lei Xu, Ya-ran Shi, Xiao-hong Yu, An Lyu, Feng-hua Liu, Lin-shu Jiang. Magnolol pretreatment attenuates heat stress-induced IEC-6 cell injury[J]. Journal of Zhejiang University Science B, 2016, 17(6): 413-424.
@article{title="Magnolol pretreatment attenuates heat stress-induced IEC-6 cell injury",
author="Chen Mei, Sha-sha He, Peng Yin, Lei Xu, Ya-ran Shi, Xiao-hong Yu, An Lyu, Feng-hua Liu, Lin-shu Jiang",
journal="Journal of Zhejiang University Science B",
volume="17",
number="6",
pages="413-424",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500261"
}
%0 Journal Article
%T Magnolol pretreatment attenuates heat stress-induced IEC-6 cell injury
%A Chen Mei
%A Sha-sha He
%A Peng Yin
%A Lei Xu
%A Ya-ran Shi
%A Xiao-hong Yu
%A An Lyu
%A Feng-hua Liu
%A Lin-shu Jiang
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 6
%P 413-424
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500261
TY - JOUR
T1 - Magnolol pretreatment attenuates heat stress-induced IEC-6 cell injury
A1 - Chen Mei
A1 - Sha-sha He
A1 - Peng Yin
A1 - Lei Xu
A1 - Ya-ran Shi
A1 - Xiao-hong Yu
A1 - An Lyu
A1 - Feng-hua Liu
A1 - Lin-shu Jiang
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 6
SP - 413
EP - 424
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500261
Abstract: Objective: heat stress (HS) is an important environmental stressor that adversely influences livestock during the summer. The aim of this study was to investigate whether magnolol protects against HS-induced intestinal epithelial cell injury. Materials and methods: An intestinal epithelial cell line (IEC-6) was subjected to HS at 42 °C, with and without magnolol pretreatment. cell injury was detected by monitoring lactate dehydrogenase (LDH) release. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was used to assess cell proliferation and viability, including identifying effective concentrations of magnolol. Flow cytometry confirmed G1-phase cell-cycle arrest and its alleviation by magnolol. Active DNA synthesis was measured by incorporation of nucleic acid 5-ethynyl-2'-deoxyuridine (EdU). G1-phase cell-cycle-related gene expression was assessed by real-time reverse transcription polymerase chain reaction (RT-PCR) and levels of G1-phase-related proteins by Western blotting. Results: HS induced IEC-6 cell injury and decreased cell viability, as demonstrated by data from LDH and MTS assays, respectively. Based on a number of criteria, IEC-6 cells subjected to HS were arrested in the G1 phase of the cell cycle. magnolol pretreatment decreased HS-induced cell injury through relief of this cell-cycle arrest. Conclusions: magnolol pretreatment attenuates HS-induced injury in IEC-6 cells. magnolol is potentially promising as a protective strategy for HS in livestock.
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