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CLC number: Q93

On-line Access: 2016-08-04

Received: 2016-04-20

Revision Accepted: 2016-05-24

Crosschecked: 2016-07-13

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Qing Gu

http://orcid.org/0000-0001-5394-6615

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.8 P.569-579

10.1631/jzus.B1600176


Comparative genomic analysis of Lactobacillus plantarum ZJ316 reveals its genetic adaptation and potential probiotic profiles


Author(s):  Ping Li, Xuan Li, Qing Gu, Xiu-yu Lou, Xiao-mei Zhang, Da-feng Song, Chen Zhang

Affiliation(s):  Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China; more

Corresponding email(s):   guqing2002@hotmail.com

Key Words:  Lactobacillus plantarum ZJ316, Comparative genomics, Probiotics, Adaptation


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Ping Li, Xuan Li, Qing Gu, Xiu-yu Lou, Xiao-mei Zhang, Da-feng Song, Chen Zhang. Comparative genomic analysis of Lactobacillus plantarum ZJ316 reveals its genetic adaptation and potential probiotic profiles[J]. Journal of Zhejiang University Science B, 2016, 17(8): 569-579.

@article{title="Comparative genomic analysis of Lactobacillus plantarum ZJ316 reveals its genetic adaptation and potential probiotic profiles",
author="Ping Li, Xuan Li, Qing Gu, Xiu-yu Lou, Xiao-mei Zhang, Da-feng Song, Chen Zhang",
journal="Journal of Zhejiang University Science B",
volume="17",
number="8",
pages="569-579",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600176"
}

%0 Journal Article
%T Comparative genomic analysis of Lactobacillus plantarum ZJ316 reveals its genetic adaptation and potential probiotic profiles
%A Ping Li
%A Xuan Li
%A Qing Gu
%A Xiu-yu Lou
%A Xiao-mei Zhang
%A Da-feng Song
%A Chen Zhang
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 8
%P 569-579
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600176

TY - JOUR
T1 - Comparative genomic analysis of Lactobacillus plantarum ZJ316 reveals its genetic adaptation and potential probiotic profiles
A1 - Ping Li
A1 - Xuan Li
A1 - Qing Gu
A1 - Xiu-yu Lou
A1 - Xiao-mei Zhang
A1 - Da-feng Song
A1 - Chen Zhang
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 8
SP - 569
EP - 579
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600176


Abstract: 
Objective: In previous studies, Lactobacillus plantarum ZJ316 showed probiotic properties, such as antimicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis. Methods: The genome sequence of L. plantarum ZJ316 was compared with those of eight L. plantarum strains deposited in GenBank. BLASTN, Mauve, and MUMmer programs were used for genome alignment and comparison. CRISPRFinder was applied for searching the clustered regularly interspaced short palindromic repeats (CRISPRs). Results: We identified genes that encode proteins related to genetic adaptation and probiotic profiles, including carbohydrate transport and metabolism, proteolytic enzyme systems and amino acid biosynthesis, CRISPR adaptive immunity, stress responses, bile salt resistance, ability to adhere to the host intestinal wall, exopolysaccharide (EPS) biosynthesis, and bacteriocin biosynthesis. Conclusions: Comparative characterization of the L. plantarum ZJ316 genome provided the genetic basis for further elucidating the functional mechanisms of its probiotic properties. ZJ316 could be considered a potential probiotic candidate.

比较基因组学揭示植物乳杆菌ZJ316的生境适 应性及潜在益生特性

目的:前期研究发现植物乳杆菌ZJ316能显著抑制病原菌,促进仔猪生长,提高猪肉质量等,本研究拟在ZJ316全基因组测序的基础上,运用比较基因组学手段揭示与其生境适应性及益生特性相关基因。
创新点:首次从基因水平上分析与植物乳杆菌ZJ316的生境适应性、抑菌活性及益生特性等相关的基因,为进一步揭示其生理功能打下基础。
方法:运用BLASTN、Mauve和MUMmer等将植物乳杆菌ZJ316全基因组序列与已测序的8个植物乳杆菌全基因组序列进行比对及分析;用CRISPRFinder寻找CRISPR重复序列。
结论:植物乳杆菌ZJ316包含碳水化合物的运输和代谢、蛋白水解酶系统和氨基酸的生物合成等相关基因,具有CRISPR、应激反应、耐胆盐、粘附宿主肠壁、胞外多糖、生物合成和细菌素生物合成等相关基因。这些基因的功能是其作为益生菌的重要特征和基础。

关键词:植物乳杆菌ZJ316;比较基因组学;益生菌;适应性

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[42]List of electronic supplementary materials

[43]Table S1 PTS predicted in L. plantarum ZJ316

[44]Table S2 Peptidases and proteases genes in L. plantarum ZJ316

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