CLC number: S821.5
On-line Access: 2016-10-02
Received: 2016-05-19
Revision Accepted: 2016-08-04
Crosschecked: 2016-09-10
Cited: 1
Clicked: 4737
Zhi-mei Tian, Xian-yong Ma, Xue-fen Yang, Qiu-li Fan, Yun-xia Xiong, Yue-qin Qiu, Li Wang, Xiao-lu Wen, Zong-yong Jiang. Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets[J]. Journal of Zhejiang University Science B, 2016, 17(10): 742-751.
@article{title="Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets",
author="Zhi-mei Tian, Xian-yong Ma, Xue-fen Yang, Qiu-li Fan, Yun-xia Xiong, Yue-qin Qiu, Li Wang, Xiao-lu Wen, Zong-yong Jiang",
journal="Journal of Zhejiang University Science B",
volume="17",
number="10",
pages="742-751",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600229"
}
%0 Journal Article
%T Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets
%A Zhi-mei Tian
%A Xian-yong Ma
%A Xue-fen Yang
%A Qiu-li Fan
%A Yun-xia Xiong
%A Yue-qin Qiu
%A Li Wang
%A Xiao-lu Wen
%A Zong-yong Jiang
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 10
%P 742-751
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600229
TY - JOUR
T1 - Influence of low protein diets on gene expression of digestive enzymes and hormone secretion in the gastrointestinal tract of young weaned piglets
A1 - Zhi-mei Tian
A1 - Xian-yong Ma
A1 - Xue-fen Yang
A1 - Qiu-li Fan
A1 - Yun-xia Xiong
A1 - Yue-qin Qiu
A1 - Li Wang
A1 - Xiao-lu Wen
A1 - Zong-yong Jiang
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 10
SP - 742
EP - 751
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600229
Abstract: To investigate dietary protein level effects on digestive mechanisms, weaned piglets were fed for 45 d with diets containing 20%, 17%, or 14% crude protein (CP) supplemented to meet requirements for essential amino acids. This article describes the influence of dietary protein on gastrointestinal hormones and expression of an array of digestive enzymes in the gastrointestinal tract and pancreas. Results indicated that there were no significant differences in expression of enzymes involved in carbohydrate digestion, except for maltase in the duodenum. In the jejunum, amylase expression in pigs fed 20% CP was much higher than that in pigs fed other diets (P<0.05) and maltase expression in those fed 17% CP was higher than that in other treatments (P<0.05). Although there were no remarkable differences in expression of aminopeptidase in the small intestine or carboxypeptidase in the pancreas (P>0.05), there was a trend towards higher expression of various proteases in pigs fed 17% CP. The duodenal expression of enteropeptidase in diets with 14% and 17% CP was significantly higher than that with 20% CP (P<0.05), but treatment differences did not existed in jejunum (P>0.05). The expression of GPR93 as a nutrient-responsive G protein-coupled receptor in 14% and 17% CP diets was significantly higher than that in 20% CP diet in the small intestine (P<0.05). The expressions of genes for pancreatic enzymes, lipase and elastase, were significantly higher in pigs fed diets with low CP, while similar trends occurred for carboxypeptidase, chymotrypsin and amylase. Conversely, the gastric expressions of pepsinogen A and progastricsin were lower with the 17% CP diet. Differences between treatments were found in the gastric antral contents of cholecystokinin and somatostatin: both increased in pigs fed 17% CP, accompanied by decreased content of motilin, which was also seen in plasma concentrations. These patterns were not reflected in duodenal contents. In general, 17% dietary CP was beneficial to the digestion of nutrient substance in the gastrointestinal tract.
[1]Awati, A., Williams, B.A., Bosch, M.W., et al., 2006. Effect of inclusion of fermentable carbohydrates in the diet on fermentation end-product profile in feces of weanling piglets. J. Anim. Sci., 84(8):2133-2140.
[2]Barea, R., Nieto, R., Aguilera, J.F., 2007. Effects of the dietary protein content and the feeding level on protein and energy metabolism in Iberian pigs growing from 50 to 100 kg body weight. Animal, 1(3):357-365.
[3]Bikker, P., Dirkzwager, A., Fledderus, J., et al., 2006. The effect of dietary protein and fermentable carbohydrates levels on growth performance and intestinal characteristics in newly weaned piglets. J. Anim. Sci., 84(12):3337-3345.
[4]Chaudhri, O., Small, C., Bloom, S., 2006. Gastrointestinal hormones regulating appetite. Philos. Trans. R. Soc. Lond. B Biol. Sci., 361(1471):1187-1209.
[5]Chen, Y., Hui, H., Yang, H., et al., 2013. Wogonoside induces cell cycle arrest and differentiation by affecting expression and subcellular localization of PLSCR1 in AML cells. Blood, 121(18):3682-3691.
[6]Choi, S., Lee, M., Shiu, A.L., et al., 2007. GPR93 activation by protein hydrolysate induces CCK transcription and secretion in STC-1 cells. Am. J. Physiol. Gastrointest. Liver Physiol., 292(5):G1366-G1375.
[7]Committee on Nutrient Requirements of Swine, National Research Council, 2012. Nutrient Requirements of Swine, 11th Revised Ed. The National Academies Press, Washington, DC.
[8]Green, G.M., Levan, V.H., Liddle, R.A., 1986. Plasma cholecystokinin and pancreatic growth during adaptation to dietary protein. Am. J. Physiol., 251(1 Pt 1):G70-G74.
[9]Hara, H., Ohyama, S., Hira, T., 2001. Endogenous cholecystokinin plays a role in down-regulation of pancreatic amylase independent of dietary carbohydrate in rats. Regul. Pept., 99(2-3):103-110.
[10]He, L., Wu, L., Xu, Z., et al., 2016. Low-protein diets affect ileal amino acid digestibility and gene expression of digestive enzymes in growing and finishing pigs. Amino Acids, 48(1):21-30.
[11]Hermes, R.G., Molist, F., Ywazaki, M., et al., 2009. Effect of dietary level of protein and fiber on the productive performance and health status of piglets. J. Anim. Sci., 87(11): 3569-3577.
[12]Htoo, J.K., Sauer, W.C., Zhang, Y., et al., 2007. The effect of feeding low-phytate barley-soybean meal diets differing in protein content to growing pigs on the excretion of phosphorus and nitrogen. J. Anim. Sci., 85(3):700-705.
[13]Jahan-Mihan, A., Luhovyy, B.L., el Khoury, D., et al., 2011. Dietary proteins as determinants of metabolic and physiologic functions of the gastrointestinal tract. Nutrients, 3(5):574-603.
[14]Jin, C.F., Kim, J.H., Han, I.K., et al., 1998. Effects of supplemental synthetic amino acids to the low protein diets on the performance of growing pigs. Asian Australas. J. Anim. Sci., 11(1):1-7.
[15]Kendall, D.C., Gaines, A.M., Allee, G.L., et al., 2008. Commercial validation of the true ileal digestible lysine requirement for eleven- to twenty-seven-kilogram pigs. J. Anim. Sci., 86(2):324-332.
[16]le Bellego, L., van Milgen, J., Noblet, J., 2002. Effect of high temperature and low-protein diets on the performance of growing-finishing pigs. J. Anim. Sci., 80(3):691-701.
[17]Len, N.T., Hong, T.T., Ogle, B., et al., 2009. Comparison of total tract digestibility, development of visceral organs and digestive tract of Mong cai and Yorkshire×Landrace piglets fed diets with different fibre sources. J. Anim. Physiol. Anim. Nutr. (Berl.), 93(2):181-191.
[18]Leray, V., Segain, J.P., Cherbut, C., et al., 2003. Adaptation to low-protein diet increases inhibition of gastric emptying by CCK. Peptides, 24(12):1929-1934.
[19]Lindemann, M.D., Cornelius, S.G., el Kandelgy, S.M., et al., 1986. Effect of age, weaning and diet on digestive enzyme levels in the piglet. J. Anim. Sci., 62(5):1298-1307.
[20]Ling, N., Burgus, R., Rivier, J., et al., 1973. The use of mass spectrometry in deducing the sequence of somatostatin— a hypothalamic polypeptide that inhibits the secretion of growth hormone. Biochem. Biophys. Res. Commun., 50(1):127-133.
[21]Lowe, M.E., 1994. Pancreatic triglyceride lipase and colipase: insights into dietary fat digestion. Gastroenterology, 107(5):1524-1536.
[22]Makkink, C.A., Berntsen, P.J., op den Kamp, B.M., et al., 1994. Gastric protein breakdown and pancreatic enzyme activities in response to two different dietary protein sources in newly weaned pig. J. Anim. Sci., 72(11):2843-2850.
[23]Moran, T.H., Kinzig, K.P., 2004. Gastrointestinal satiety signals II. Cholecystokinin. Am. J. Physiol. Gastrointest. Liver Physiol., 286(2):G183-G188.
[24]Morisset, J., Wong, H., Walsh, J.H., et al., 2000. Pancreatic CCK(B) receptors: their potential roles in somatostatin release and delta-cell proliferation. Am. J. Physiol. Gastrointest. Liver Physiol., 279(1):G148-G156.
[25]Nilaweera, K.N., Giblin, L., Ross, R.P., 2010. Nutrient regulation of enteroendocrine cellular activity linked to cholecystokinin gene expression and secretion. J. Physiol. Biochem., 66(1):85-92.
[26]Nyachoti, C.M., Omogbenigun, F.O., Rademacher, M., et al., 2006. Performance responses and indicators of gastrointestinal health in early-weaned pigs fed low-protein amino acid-supplemented diets. J. Anim. Sci., 84(1):125-134.
[27]Patel, Y.C., 1999. Somatostatin and its receptor family. Front. Neuroendocrinol., 20(3):157-198.
[28]Peng, X., Hu, L., Liu, Y., et al., 2016. Effects of low-protein diets supplemented with indispensable amino acids on growth performance, intestinal morphology and immunological parameters in 13 to 35 kg pigs. Animal, first view.
[29]Pinheiro, D.F., Pacheco, P.D., Alvarenga, P.V., et al., 2013. Maternal protein restriction affects gene expression and enzyme activity of intestinal disaccharidases in adult rat offspring. Braz. J. Med. Biol. Res., 46(3):287-292.
[30]Shi, G., Leray, V., Scarpignato, C., et al., 1997. Specific adaptation of gastric emptying to diets with differing protein content in the rat: is endogenous cholecystokinin implicated? Gut, 41(5):612-618.
[31]Wank, S.A., 1995. Cholecystokinin receptors. Am. J. Physiol., 269(5 Pt 1):G628-G646.
[32]Williams, J.A., 2001. Intracellular signaling mechanisms activated by cholecystokinin-regulating synthesis and secretion of digestive enzymes in pancreatic acinar cells. Annu. Rev. Physiol., 63:77-97.
[33]Wu, L., He, L.Q., Cui, Z.J., et al., 2015. Effects of reducing dietary protein on the expression of nutrition sensing genes (amino acid transporters) in weaned piglets. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 16(6):496-502.
[34]Xiao, X., Ross, L.E., Sevilla, W.A., et al., 2013. Porcine pancreatic lipase related protein 2 has high triglyceride lipase activity in the absence of colipase. Biochim. Biophys. Acta, 1831(9):1435-1441.
[35]Yang, W., Wang, J., Liu, L., et al., 2011. Effect of high dietary copper on somatostatin and growth hormone-releasing hormone levels in the hypothalami of growing pigs. Biol. Trace Elem. Res., 143(2):893-900.
[36]Yue, L.Y., Qiao, S.Y., 2008. Effects of low-protein diets supplemented with crystalline amino acids on performance and intestinal development in piglets over the first 2 weeks after weaning. Livest. Sci., 115(2-3):144-152.
Open peer comments: Debate/Discuss/Question/Opinion
<1>