CLC number: R284.2
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2017-07-19
Cited: 0
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Citations: Bibtex RefMan EndNote GB/T7714
Yun Chen, Xiu-ju Du, Yang Zhang, Xin-hua Liu, Xuan-dong Wang. Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake[J]. Journal of Zhejiang University Science B, 2017, 18(8): 674-684.
@article{title="Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake",
author="Yun Chen, Xiu-ju Du, Yang Zhang, Xin-hua Liu, Xuan-dong Wang",
journal="Journal of Zhejiang University Science B",
volume="18",
number="8",
pages="674-684",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600239"
}
%0 Journal Article
%T Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake
%A Yun Chen
%A Xiu-ju Du
%A Yang Zhang
%A Xin-hua Liu
%A Xuan-dong Wang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 8
%P 674-684
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600239
TY - JOUR
T1 - Ultrasound extraction optimization, structural features, and antioxidant activity of polysaccharides from Tricholoma matsutake
A1 - Yun Chen
A1 - Xiu-ju Du
A1 - Yang Zhang
A1 - Xin-hua Liu
A1 - Xuan-dong Wang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 8
SP - 674
EP - 684
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600239
Abstract: An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholoma matsutake fruiting bodies. Single-factor tests and orthogonal experimental design (L9(33)) were used to obtain the optimal extraction conditions. Results showed that the optimal parameters were as follows: ultrasonic temperature, 40 °C; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 kHz; and ultrasonic power, 100 W. Three novel T. matsutake polysaccharide (TMP) fractions (TMP30, TMP60, and TMP80) were isolated and purified from TMP by stepwise alcohol precipitation. Their preliminary structural features were determined by high-performance anion-exchange chromatography with pulsed-amperometric detection (HPAEC-PAD) and Fourier transform infrared spectrophotometer (FT-IR) analyses. Furthermore, their in vitro antioxidant activity was investigated in terms of a reducing power assay and the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals. The order of the various fractions based on their antioxidant activity was TMP80>TMP>TMP60>TMP30. These findings suggested that novel polysaccharide fractions from T. matsutake, especially TMP80, could be promising active macromolecules for biomedical use.
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