CLC number: R961.1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2017-06-07
Cited: 0
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Li-qin Wang, Yu He, Hao-fang Wan, Hui-fen Zhou, Jie-hong Yang, Hai-tong Wan. Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury[J]. Journal of Zhejiang University Science B, 2017, 18(7): 586-596.
@article{title="Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury",
author="Li-qin Wang, Yu He, Hao-fang Wan, Hui-fen Zhou, Jie-hong Yang, Hai-tong Wan",
journal="Journal of Zhejiang University Science B",
volume="18",
number="7",
pages="586-596",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600270"
}
%0 Journal Article
%T Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury
%A Li-qin Wang
%A Yu He
%A Hao-fang Wan
%A Hui-fen Zhou
%A Jie-hong Yang
%A Hai-tong Wan
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 7
%P 586-596
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600270
TY - JOUR
T1 - Protective mechanisms of hypaconitine and glycyrrhetinic acid compatibility in oxygen and glucose deprivation injury
A1 - Li-qin Wang
A1 - Yu He
A1 - Hao-fang Wan
A1 - Hui-fen Zhou
A1 - Jie-hong Yang
A1 - Hai-tong Wan
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 7
SP - 586
EP - 596
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600270
Abstract: This study investigated the protective effect of the compatibility of hypaconitine (HA) and glycyrrhetinic acid (GA) on h9c2 cells under oxygen and glucose deprivation (OGD)-induced injury, and the possible mechanisms. We found that HA+GA significantly improved pathology and morphology of the nucleus and ultrastructure of h9c2 cells under OGD as determined by Hoechst 33342 staining and transmission electron microscopy (TEM) tests. It also reduced the releases of lactate dehydrogenase (LDH), creatine kinase-myocardial band isoenzyme (CK-MB), and aspartate transaminase (AST) from the cultured supernatant of h9c2 cells, which were tested by enzyme-linked immune sorbent assay (ELISA) kits. In addition, it lessened the apoptotic rate as determined by a fluorescein isothiocyanate-annexin V/propidium iodide (FITC-AV/PI) double staining assay. It was also found that HA+GA might regulate the protein expression associated with the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. Overall, the study demonstrated that HA+GA protected h9c2 cells against OGD-induced injury, and the signaling mechanism might be related to the PI3K/Akt signaling pathway.
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